Anti-IL1RA Antibody

Cat: 10123-MM02
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Anti-IL1RA Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-IL1RA Antibody
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human IL1RA
Immunogen
Recombinant Human IL-1RA protein (Catalog#10123-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-1RA (rh IL-1RA; Catalog#10123-HNAE; NP_776214.1; Arg26-Glu177). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #02
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IL1RA Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IL1RA Antibody (Mouse Monoclonal antibody) Images
Human IL-1RA/IL1RN Immunofluorescence(IF) 2905
Immunofluorescence staining of Human IL1Ra in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IL1Ra monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Human IL-1RA/IL1RN Flow Cytometry (FC) 5128
Flow cytometric analysis of Human IL1RA expression on A431 cells. Cells were stained with purified anti-Human IL1RA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-IL1RA Antibody Alternative Names
Anti-DIRA Antibody;Anti-ICIL-1RA Antibody;Anti-IL-1ra Antibody;Anti-IL-1ra3 Antibody;Anti-IL-1RN Antibody;Anti-IL1F3 Antibody;Anti-IL1RA Antibody;Anti-IRAP Antibody;Anti-MVCD4 Antibody
IL1RA Background Information

Interleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein encoding gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. Genetic variation in IL-1RA/IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in IL-1RA/IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) which is also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis, and pustulosis from birth.

Full Name
interleukin 1 receptor antagonist
References
  • Langdahl BL, et al. (2000) Osteoporotic fractures are associated with an 86-base pair repeat polymorphism in the interleukin-1--receptor antagonist gene but not with polymorphisms in the interleukin-1beta gene. J Bone Miner. 15 (3): 402-14.
  • El-Omar EM, et al. (2000) Interleukin-1 polymorphisms associated with increased risk of gastric cancer. Nature. 404 (6776): 398-402.
  • Steinkasserer A, et al. (1992) The human IL-1 receptor antagonist gene (IL1RN) maps to chromosome 2q14-q21, in the region of the IL-1 alpha and IL-1 beta loci. Genomics. 13 (3): 654-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IL1RA Antibody (FITC) Mouse Monoclonal FCM 10123-MM02-F
    Anti-IL1RA Antibody Rabbit Monoclonal ELISA 10123-R001
    Anti-IL1RA Antibody Rabbit Polyclonal ELISA 10123-RP01
    Anti-IL1RA Antibody (FITC) Rabbit Monoclonal FCM 10123-R004-F
    Anti-IL1RA Antibody Rabbit Polyclonal ELISA,IHC-P 10123-RP02
    Anti-IL1RA Antibody (PE) Mouse Monoclonal FCM 10123-MM02-P
    Anti-IL1RA Antibody Rabbit Monoclonal IHC-P 100099-R005
    Anti-IL1RA Antibody Mouse Monoclonal ELISA 10123-MM12
    Anti-IL1RA Antibody Rabbit Monoclonal FCM,ICC/IF,IF 10123-R004
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