Mouse IL-1R8 / IL1RAPL1 Protein (Cytokine)

Interleukin 1 receptor accessory protein-like 1 Protein Datasheet

IL-1R8 / IL1RAPL1 Protein Product Information

• Synonym: RP23-301L16.1, 6330532G10Rik, C030039N24, IL1R8, IL1RAPL, MRX34, OPHN4, TIGIRR-2
• Protein Construction: A DNA sequence encoding the mouse IL1RAPL1 (P59823) extracellular domain (Met 1-Thr 357) was fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

IL-1R8 / IL1RAPL1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Arg 25
• Molecular Mass: The secreted recombinant mouse IL1RAPL1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 574 amino acids and has a calculated molecular mass of 65 kDa. As a result of glycosylation, the apparent molecular mass of rmIL1RAPL1/Fc monomer is approximately 85-95 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL-1R8 / IL1RAPL1 protein

IL-1R8 / IL1RAPL1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-1R8 / IL1RAPL1 Protein Description

IL1-receptor accessory protein-like 1 (IL1RAPL1), also known as IL1R8, a member of interleukin-1/toll like receptor (TIR) family, is an integral membrane protein responsible for a nonsyndromic form of mental retardation (MR). The protein structurally comprises three extracellular immunoglobulin domains, which presumably mediate binding of an as yet unidentified ligand, a transmembrane region, and an intracellular domain, which is likely to enable signalling via the NFkB pathway. The means of signalling is almost certain to be identical to that used by the IL1R family and the more distally related Toll protein. L1RAPL1 protein physically interacts via its 150 aa C-terminal domain with neuronal calcium sensor-1 (NCS-1), a protein widely expressed in neurons and the related chromaffin and PC12 cells. IL1RAPL1 is suggested to affect human cognitive ability to some extent, especially the memory and concentration capability.

References

1. Tomoyuki Yoshida. et al., 2008, Molecular and Cellular Neuroscience. 39 (2): 218-28.
2. Frédéric Gambino. et al., 2007, Proc Natl Acad Sci. 104 (21): 9063-8.
3. Wheway JM. et al., 2003, J Med Genet. 40: 127-31.
4. Hong Jin. et al., 2000, European Journal of Human Genetics. 8: 87-94.