IL-1R8 / IL1RAPL1 Antibody (Cytokine)

IL-1R8 / IL1RAPL1 Antibody Datasheet

IL-1R8 / IL1RAPL1 Antibody Product Information

• Immunogen: Recombinant Human IL-1R8 / IL1RAPL1 protein (Catalog#10177-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 2E10E1F5
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-1R8 / IL1RAPL1 (rh IL-1R8 / IL1RAPL1; Catalog#10177-H08H; NP_055086.1; Met 1-Leu 354). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

IL-1R8 / IL1RAPL1 Antibody Usage Guide

• Specificity: Human IL-1R8 / IL1RAPL1
• No cross-reactivity in ELISA with

  Human IL18R1

  Human IL1R1

  Human IL1R2

  Human SIGIRR

  Human IL1R4

  Human IL1R7

  Human IL1RA

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human IL1R8 in WB. Using a DAB detection system, the detection limit for Human IL1R8 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human IL1R8. The detection limit for Human IL1R8 is approximately 0.625 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL-1R8 / IL1RAPL1 Antibody Background

IL1-receptor accessory protein-like 1 (IL1RAPL1), also known as IL1R8, a member of interleukin-1/toll like receptor (TIR) family, is an integral membrane protein responsible for a nonsyndromic form of mental retardation (MR). The protein structurally comprises three extracellular immunoglobulin domains, which presumably mediate binding of an as yet unidentified ligand, a transmembrane region, and an intracellular domain, which is likely to enable signalling via the NFkB pathway. The means of signalling is almost certain to be identical to that used by the IL1R family and the more distally related Toll protein. L1RAPL1 protein physically interacts via its 150 aa C-terminal domain with neuronal calcium sensor-1 (NCS-1), a protein widely expressed in neurons and the related chromaffin and PC12 cells. IL1RAPL1 is suggested to affect human cognitive ability to some extent, especially the memory and concentration capability.

References

1. Tomoyuki Yoshida, et al., 2008, Molecular and Cellular Neuroscience. 2: 218-228.
2. Frederic Gambino, et al., 2007, Proc Natl Acad Sci U S A, 104 (21): 9063-9068.
3. J M Wheway, et al., 2003, J Med Genet, 40: 127-131.
4. Hong Jin, et al., 2000, European Journal of Human Genetics, 8: 87-94.