|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
IL1R1 was immunoprecipitated using:
Lane A:0.5 mg Raw264.7 Whole Cell Lysate2 µL anti-IL1R1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-IL1R1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 65 kDa
Observed band size: 65 kDa
Anti-IL1R1 rabbit polyclonal antibody at 1:500 dilution
Lane A: RAW264.7Membrane LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:65 kDa
Observed band size:65 kDa
(We are unsure as to the identity of these extra bands.)
Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-IL1R1 Antibody (PE)||Rabbit||Monoclonal||FCM||80028-R007-P|
|Anti-IL1R1 Antibody (FITC)||Rabbit||Monoclonal||FCM||80028-R007-F|
|Anti-IL1R1 Antibody (APC)||Rabbit||Monoclonal||FCM||50807-R191-A|
|Anti-IL1R1 Antibody (PE)||Rabbit||Monoclonal||FCM||50807-R191-P|