IL-1F6 Antibody (Antigen Affinity Purified)

IL-1F6 Antibody ( Antigen Affinity Purified ) Datasheet

IL-1F6 Antibody Product Information

• Immunogen: Recombinant Mouse IL-1F6 protein (Catalog#50095-HNAE)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse IL-1F6 (rM IL-1F6; Catalog#50095-HNAE; Q9JLA2-1; Met 1-His 160). IL-1F6 specific IgG was purified by Mouse IL-1F6 affinity chromatography.

IL-1F6 Antibody Usage Guide

• Specificity: Mouse IL-1F6
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IL1F6 in WB. Using a DAB detection system, the detection limit for Mouse IL1F6 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IL1F6. The detection limit for Mouse IL1F6 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL-1F6 Antibody Background

Interleukin-1 family member 6, also known as IL-1F6, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, IL1F6 and FIL1E, is a secreted protein which belongs to the IL-1 family. IL-1F6 is a new IL-1 family member. IL1F6 / FIL1E is expressed in immune system, fetal brain and adipose tissue and that IL-1F6 and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-1F6 inhibits PPARγ expression which may lead to reduced adipocyte differentiation suggesting metabolic effects of this cytokine.. IL1F6 / FIL1E, along with IL-36β / IL-1F8 and IL-36γ / IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-κB and MAPK in an IL-1 Rrp2 dependent manner. IL1F6 / FIL1E may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References

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5. Dinarello C. et al., 2010, Nat Immunol. 11: 973.