Anti-IL-18 Antibody

Cat: 90011-RP02
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All IL-18 Reagents
Anti-IL-18 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-IL-18 Antibody
Validated applications
Species reactivity
Reacts with: Rhesus
Rhesus IL-18
Recombinant Rhesus IL18 / Interleukin-18 protein (Catalog#90011-C07E)
Produced in rabbits immunized with purified, recombinant Rhesus IL18 / Interleukin-18 (Catalog#90011-C07E; NP_001028006.1; Ala2-Asp193). IL18 / Interleukin-18 specific IgG was purified by Rhesus IL18 / Interleukin-18 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IL-18 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IL-18 Antibody (Rabbit Polyclonal antibody) Images
Cynomolgus IL18/IL-18/Interleukin 18 Immunoprecipitation(IP) 6107

cyno IL18 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-cyno IL18 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-cyno IL18 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 22 kDa

Observed band size: 22 kDa

Cynomolgus IL18/IL-18/Interleukin 18 Western blot (WB) 13756

Anti-IL18 rabbit polyclonal antibody at 1:500 dilution

Lane A: HL-60 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:22 kDa

IL-18 Background Information

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
interleukin 18
  • Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24.
  • Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90.
  • Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IL-18 Antibody Mouse Monoclonal ELISA 90011-MM02
    Anti-IL-18 Antibody Mouse Monoclonal ELISA 90011-MM17
    Anti-IL-18 Antibody Rabbit Polyclonal ELISA 90011-RP01
    Anti-IL-18 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 50073-R167
    Anti-IL-18 Antibody Rabbit Polyclonal WB,ELISA 50073-T48
    Anti-IL-18 Antibody Rabbit Monoclonal ELISA(Cap) 50073-R169
    Anti-IL-18 Antibody Rabbit Polyclonal ELISA 50073-T16
    Anti-IL-18 Antibody Rabbit Polyclonal ELISA 50073-RP01
    Anti-IL-18 Antibody Mouse Monoclonal ELISA 10119-MM15
    Anti-IL-18 Antibody Rabbit Polyclonal ELISA 10119-T16
    Anti-IL-18 Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 10119-R011
    Anti-IL-18 Antibody Rabbit Polyclonal WB,ELISA,IP 10119-T52
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