IL-16 Antibody (Cytokine)

IL-16 Antibody Datasheet

IL-16 Antibody Product Information

• Immunogen: Recombinant Human IL-16 protein (Catalog#10944-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human IL-16 (rh IL-16; Catalog#10944-H07E; AAC12732.1; Pro 2-Ser 130). Total IgG was purified by Protein A affinity chromatography.

IL-16 Antibody Usage Guide

• Specificity: Human IL-16
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human IL16 in WB.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human IL16. The detection limit for Human IL16 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL-16 Antibody Background

Interleukin-16, also known as Lymphocyte chemoattractant factor, LCF and IL16, is a secreted, cytoplasm and nucleus protein which contains 4PDZ (DHR) domains. Interleukin-16 / IL16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF). Since then, this interleukin has been shown to recruit and activate many other cells expressing the CD4 molecule, including monocytes, eosinophils, and dendritic cells. The structure of Interleukin-16 / IL16 was determined following its cloning in 1994. Interleukin-16 / IL16 is produced as a precursor peptide (pro-IL-16) that requires processing by an enzyme called caspase-3 to become active. CD4 is the cell signaling receptor for mature IL-16. Interleukin-16 / IL16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. Interleukin-16 / IL16 is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. The signaling process of Interleukin-16 / IL16 is mediated by CD4. It undergoes proteolytic processing, which is found to yield two functional proteins. Interleukin-16 / IL16 function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.

References

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