Mouse IL-13 / Interleukin-13 Protein (Native) (Cytokine)

Interleukin 13 Protein Datasheet

IL-13 / Interleukin-13 Protein Product Information

• Synonym: IL13, Il-13
• Protein Construction: A DNA sequence encoding the mouse IL13 (P20109) (Pro22-Phe131) was expressed and purified.
• Source: Mouse
• Expression Host: Human Cells

IL-13 / Interleukin-13 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Pro 22
• Molecular Mass: The recombinant mouse IL13 consists of 110 amino acids and predicts a molecular mass of 12.1 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.5.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL-13 / Interleukin-13 protein

IL-13 / Interleukin-13 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-13 / Interleukin-13 Protein Description

Interleukin-13, also known as IL-13 and NC30, is a secreted protein which belongs to the IL-4 / IL-13 family It is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. Interleukin-13 / IL-3 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL13 is more importantly implicated as a central mediator of immunoregulatory processes in various cell types. Interleukin-13 / IL-3 is involved in several stages of B-cell maturation and differentiation including up-regulating CD23, CD71, CD72, and MHC class II expression, and promoting IgE isotype switching. Interleukin-13 / IL-3 inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. Interleukin-13 / IL-3 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL13 Rα1 and IL13 Rα2). Most of the biological effects of Interleukin-13 / IL-3, like those of IL-4, are linked to signal transducer and activator of transcription 6 (STAT6). Interleukin-13 / IL-3 is associated primarily with the pathophysiology of atopic respiratory diseases such as allergic asthma. In addition, studies have identified IL13 expression as a common feature of cHL (classical Hodgkin lymphoma). Defects in IL-13 may be a cause of susceptibility to allergic rhinitis (ALRH) which is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.

References

1. Minty A. et al., 1993, Nature. 362: 248-50.
2. Sironi M. et al., 1994, Blood. 84: 1913-21.
3. Solymar DC. et al., 2002, Immunity. 17: 41-50.
4. Skinnider BF. et al., 2002, Leuk Lymphoma. 43: 1203-10.
5. Monticelli S. et al., 2004, J Biol Chem. 279: 36210-8.