A DNA sequence encoding the mouse IL13 (P20109) (Pro22-Phe131) was expressed and purified.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
MouseIL13/ALRHProtein QC Testing
> 90 % as determined by SDS-PAGE
Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.). The ED50 for this effect is typically 2-10 ng/mL.
< 1.0 EU per μg of the protein as determined by the LAL method
Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:
The recombinant mouse IL13 consists of 110 amino acids and predicts a molecular mass of 12.1 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements.
MouseIL13/ALRHProtein Usage Guide
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.
Junttila IS, et al. (2008) Tuning sensitivity to IL-4 and IL-13: differential expression of IL-4Ralpha, IL-13Ralpha1, and gammac regulates relative cytokine sensitivity. J Exp Med. 205(11): 2595-608.
Shimamura T,et al. (2008) Novel role of IL-13 in fibrosis induced by nonalcoholic steatohepatitis and its amelioration by IL-13R-directed cytotoxin in a rat model. J Immunol. 181(7): 4656-65.