Mouse IL10Rb Gene cDNA Clone / ORF Clone

IL10RB cDNA Clone Datasheet

IL10RB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008349.4
• cDNA Size: 1057
• Gene Synonym: Crfb4; CRF2-4; Il10r2; D21S58h; IL-10R2; AI528744; D16H21S58; 6620401D04Rik
• Source: Mouse

IL10RB cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus interleukin 10 receptor, beta DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 2682 C/T not causing the amino acid variation.

IL10RB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL10RB cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL10RB cDNA Clone Sequence and Quality Control

Sequence:

1        LATGTCCTGG GCGCCGAGCG TGGCGGGCTG GCTGGGTGGC TTCCTTCTGG TGCCAGCTCT
61       AGGAATGATT CCACCCCCTG AGAAGGTCAG AATGAATTCA GTTAATTTCA AGAACATTCT
121      ACAGTGGGAG GTACCTGCTT TCCCCAAAAC GAACCTGACT TTCACAGCTC AGTATGAAAG
181      TTACAGGTCT TTCCAAGATC ACTGCAAGCG CACTGCCTCG ACTCAGTGCG ACTTCTCTCA
241      TCTTTCTAAA TACGGAGACT ACACTGTGAG AGTCAGGGCT GAATTGGCGG ATGAACATTC
301      GGAGTGGGTC AATGTCACCT TCTGCCCCGT GGAAGACACC ATCATTGGAC CTCCTGAGAT
361      GCAGATAGAA TCCCTTGCTG AGTCTTTACA CCTGCGTTTC TCAGCCCCAC AAATTGAGAA
421      TGAGCCTGAG ACGTGGACCT TGAAGAACAT TTATGACTCA TGGGCTTACA GAGTGCAATA
481      CTGGAAAAAT GGGACTAATG AGAAGTTTCA AGTTGTGTCT CCGTACGACT CTGAGGTCCT
541      CCGGAACCTG GAGCCGTGGA CAACTTACTG CATTCAAGTT CAAGGGTTTC TTCTCGACCA
601      GAACAGAACA GGAGAGTGGA GTGAACCCAT CTGTGAACGG ACAGGCAATG ACGAAATAAC
661      CCCTTCCTGG ATTGTGGCCA TCATCCTCAT AGTCTCCGTC CTGGTGGTCT TCCTCTTCCT
721      CCTGGGCTGC TTTGTCGTGC TGTGGCTCAT TTATAAGAAG ACCAAGCATA CCTTCCGTTC
781      TGGGACGTCT CTTCCACAGC ACCTGAAGGA GTTTCTGGGC CACCCCCATC ACAGCACGTT
841      TCTGCTGTTC TCCTTCCCTC CCCCCGAGGA GGCCGAGGTG TTCGACAAAC TAAGCATCAT
901      CAGCGAAGAG TCTGAAGGCA GCAAGCAGAG TCCTGAAGAC AACTGTGCCT CAGAGCCCCC
961      GTCTGATCCA GGGCCTCGGG AGCTGGAGTC CAAGGATGAA GCTCCCTCAC CTCCACACGA
1021     TGACCCCAAA CTGCTCACGT CGACCTCAGA AGTATGA

 

Quality Control:cDNA sequencing results is available upon request.