Human IL10 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10947-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_000572.2

cDNA Size:   537

Synonym:  CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens interleukin 10 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGCACAGCT CAGCACTGCT CTGTTGCCTG GTCCTCCTGA CTGGGGTGAG GGCCAGCCCA
61       GGCCAGGGCA CCCAGTCTGA GAACAGCTGC ACCCACTTCC CAGGCAACCT GCCTAACATG
121      CTTCGAGATC TCCGAGATGC CTTCAGCAGA GTGAAGACTT TCTTTCAAAT GAAGGATCAG
181      CTGGACAACT TGTTGTTAAA GGAGTCCTTG CTGGAGGACT TTAAGGGTTA CCTGGGTTGC
241      CAAGCCTTGT CTGAGATGAT CCAGTTTTAC CTGGAGGAGG TGATGCCCCA AGCTGAGAAC
301      CAAGACCCAG ACATCAAGGC GCATGTGAAC TCCCTGGGGG AGAACCTGAA GACCCTCAGG
361      CTGAGGCTAC GGCGCTGTCA TCGATTTCTT CCCTGTGAAA ACAAGAGCAA GGCCGTGGAG
421      CAGGTGAAGA ATGCCTTTAA TAAGCTCCAA GAGAAAGGCA TCTACAAAGC CATGAGTGAG
481      TTTGACATCT TCATCAACTA CATAGAAGCC TACATGACAA TGAAGATACG AAACTGA

Quality Control: cDNA sequencing results is available upon request.

IL-10 related areas, pathways, and other information

Cytokine