IL-1 alpha / IL-1F1 / IL1A Antibody (Cytokine)

IL-1 alpha / IL-1F1 / IL1A Antibody Datasheet

IL-1 alpha / IL-1F1 / IL1A Antibody Product Information

• Immunogen: Recombinant Mouse IL-1 alpha / IL-1F1 / IL1A protein (Catalog#50114-MNAE)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 024
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: 3This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IL-1 alpha / IL-1F1 / IL1A (rM IL-1 alpha / IL-1F1 / IL1A; Catalog#50114-MNAE; NP_034684.2; Ser 115-Ser 270).

IL-1 alpha / IL-1F1 / IL1A Antibody Usage Guide

• Specificity: Mouse IL-1 alpha / IL-1F1 / IL1A
• No cross-reactivity in ELISA with

  Human IL1A

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse IL1A in WB.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IL1A. The detection limit for Mouse IL1A is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL-1 alpha / IL-1F1 / IL1A Antibody Background

Interleukin 1 (IL1) is an important cytokine mediator involved in a variety of biological processes including immune responses, inflammatory, and promoting tissue degradation. IL1 comprises two proteins, designated as IL-1 alpha/IL1α and IL-1 beta/IL1β which are encoded by distinct genes, and bind the same surface receptors with high affinity. IL1 proteins are synthesized as 31 kDa cytosolic precursors in monocytes and macrophages without signal peptides, and are proteolytically processed and released as 17kDa mature molecules in response to various stimulus not via the classical secretory pathway. It has been reported that release of IL1 is efficiently induced by cell injury. IL-1 alpha/L1α is released as a mixture of unprocessed and processed molecules cleaved by a calpain-like protease, and both of the forms show full biological activity. Elevated levels of IL-1 alpha/IL1α have been associated with a number of chronic disorders, including rheumatoid arthritis and Alzheimer's disease, and therefore, may provide novel targets for therapeutic approaches aimed at controlling inflammatory processes.

References

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2. Hogguist, K.A. et al., 1991, Proc. Natl. Acad. Sci. 88: 8485-8489.
3. Griffin, W.S. et al. 2002, J. Leukoc. Biol. 72: 233-238.
4. Perreqaux, D.G. et al., 1998, J. Immunol. 160: 2469-2477.
5. Brunn, G.J. et al., 2008, Circ. Res. 102: 823-830.