Rat IGSF8 Gene cDNA Clone / ORF Clone

IGSF8 cDNA Clone Datasheet

IGSF8 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001014787.1
• cDNA Size: 1836
• Gene Synonym: MGC105805; Igsf8
• Source: Rat

IGSF8 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus immunoglobulin superfamily, member 8 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 201 C/T and 1459 C/T not causing the amino acid variation.

IGSF8 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IGSF8 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IGSF8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCGTCC CTAGCCCCAC GCCGCTGAGT TCGCTGCTGC TGCTGCTGCT AATACTTGGA
61       GCCAGGTGCT ACGCCCGGCA GGTGCACGTC CCCAAGGGGC CTCTTTACCG TGTGGCTGGC
121      ACCGCTGTCT CTATCTCCTG CAATGTGAGT GACTATGAGG GCCCTTCCCA GCAAGACTTC
181      GAGTGGTTCA TGTACAGACC TGAGGCCCCA GCTACGTCAC TAGGCATCAT CAGCACCAAG
241      GACAGCCAGT TCTCCTATGC TGTCTTTGGG CCTCGAGTGG CATCTGGTGA CCTGCAGGTG
301      CAGCGCCTGA AGGGGGACTC CGTGGTGCTC AAGATTGCTC GCCTGCAGGC CCAGGACTCT
361      GGCTTTTATG AGTGCTACAC CCCCTCTACG GATACTCAGT ACCTGGGCAA CTACAGTGCC
421      AAGGTGGAGC TGAGAGTTCT TCCAGATGAG CTGCAGGTAT CTGCTGCCCC TCCAGGGCCC
481      CGAGGACGCC AGGTGGCAAC CTCCCCGTCA CGCCTCACAG TACATGAGGG GCAAGAGCTG
541      GCGCTGGGCT GCCTGGCTCA GACCAAAACG AAGAAACACA CACACCTGTC GGTGTCTTTT
601      GGGAGAGCCA TTCCTGAGGC GCCAGTGGGG CGAGCCACCC TACAGGAAGT AGTGGGACTG
661      CGCTCTGACA TGGCTGTGGA GGCCGGAGCT CCCTATGCTG AGAGGCTGGT TGCCGGGGAG
721      CTTCGGCTGA GCAAGGAAGG GACTGATCGG TACCGCATGG TGGTTGGGGG TGCCCAGGCT
781      GGAGACTCCG GCACCTACCA CTGCACTGCC GCTGAGTGGA TTCAAGATCC TGATGGCTCC
841      TGGGTTCAGG TCGCAGAGAA GAGGGCAGTC CTGGCTCATG TGGATGTGCA GACACTGTCC
901      AGCCAGCTAG CAGTGACAGT GGGACCTGGT GAACGGCGGA TTGGCCCAGG GGAACCCTTG
961      GAACTGCTGT GTAATGTGTC GGGGGCATTG CCCCCAGCAG GCCGTCATGC TGCATACTCT
1021     GTGGGCTGGG AGATGGCCCC TGCAGGGGCT CCAGGGCCTG GACGCCTGGT GGCCCAGCTG
1081     GACACAGAAG GTATAGGCAG CCTGGGCCCT GGCTATGAAG ACAGGCACAT TGCCATGGAG
1141     AAAGTAGCAT CCAGAACCTA CCGACTACGG CTGGAGGCTG CCAGGCCCGC TGACGCGGGC
1201     ACCTACCGCT GCCTCGCCAA AGCCTACGTT CGAGGCTCTG GGACCCGGCT TCGTGAAGCA
1261     GCCAGTGCTC GTTCCCGGCC TCTCCCGGTG CATGTGAGAG AAGAAGGCGT GGTGCTAGAG
1321     GCCGTGGCGT GGCTAGCAGG GGGCACTGTG TACCGGGGAG AGACCGCCTC CCTGCTGTGT
1381     AACATCTCTG TGCGGGGCGG CCCCCCGGGG CTGCGACTAG CAGCCAGCTG GTGGGTGGAG
1441     AGACCAGAGG AGGGCGAATT GAGCTCTGGT CCTGCTCAGC TTGTGGGTGG AGTGGGTCAG
1501     GACGGTGTGG CAGAGCTGGG AGTCCGGCCT GGAGGGAGTC CTGTCAGTGT GGAGCTGGTG
1561     GGACCCAGAA GTCATCGGCT GAGACTGCAC AGCTTGGGGC CCGAGGACGA AGGCATATAC
1621     CACTGTGCGC CCAGCGCCTG GGTGCAGCAC GCTGACTATA GCTGGTACCA GGCAGGCAGT
1681     GCGCGCTCCG GGCCCGTCAC GGTCTACCCC TACACGCATG CTCTGGATAC CCTGTTTGTG
1741     CCCTTGTTGG TGGGTACAGG AGTCGCCCTA GTTACGGGAG CCAGCATCCT CGCTACCATC
1801     ACCTGCTGTT TTATGAAGAG GCTGAGGAAG CGGTGA

 

Quality Control:cDNA sequencing results is available upon request.