Mouse IGSF8 Gene cDNA Clone / ORF Clone

IGSF8 cDNA Clone Datasheet

IGSF8 cDNA Clone Product Information

• Gene Bank Ref.ID: AF439263.1
• cDNA Size: 1836
• Gene Synonym: PGRL; EWI-2; KCT-4; ESTM34; R75384; AA033172; AU041109; Igsf8
• Source: Mouse

IGSF8 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus immunoglobulin superfamily, member 8 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1137 G/A resulting in the amino acid Met substitution by Ile.

IGSF8 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IGSF8 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IGSF8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCGTCC CTAGCCCCAC GCCGCTGAGT TCGCTCCTGC TGCTGCTGCT AATACTTGGA
61       ACCAGGTGCT ACGCCCGGCA GGTGCATGTC CCCAGGGGAC CTCTTTACCG GGTGGCTGGC
121      ACCGCTGTCT CTATCTCCTG CAACGTGAGT GACTATGAGG GCCCTGCCCA GCAAGACTTC
181      GAGTGGTTCA TGTACAGACC AGAGGCCCCA GCTACGTCCC TGGGCATTGT CAGCACCAAG
241      GATAGCCAGT TCTCCTATGC TGTCTTTGGG CCTCGTGTGG CATCTGGTGA CCTGCAGGTG
301      CAGCGCCTGA AGGGAGATTC GGTGGTGCTC AAGATTGCTC GCCTGCAGGC CCAGGACTCT
361      GGCTTTTATG AGTGCTACAC CCCCTCCACA GATACGCAGT ACCTGGGCAA CTACAGTGCC
421      AAGGTGGAGC TGAGAGTTCT TCCAGATGAG CTGCAGGTAT CTGCTGCCCC TCCAGGGCCC
481      CGAGGGCGCC AGGCTGCCAC CTCCCCCTCA CGCCTCACAG TGCACGAGGG GCAGGAGCTT
541      GCGCTGGGCT GCCTGGCTCA GACCAAAACA AAGAAACACA CACACCTGTC GGTGTCCTTT
601      GGGAGAGCCA TTCCGGAGGC GCCAGTGGGG CGAGCCACTC TACAGGAAGT AGTGGGACTG
661      CACTCCGACA TGGCGGTGGA GGCTGGAGCG CCCTACGCTG AGAGGCTAGC TTCTGGGGAG
721      CTTCGGCTGA GCAAGGAAGG GACTGATCGG TACCGAATGG TGGTTGGGGG TGCCCAGGCT
781      GGAGACTCGG GCACCTACCA CTGTACGGCC GCTGAGTGGA TTCAGGATCC TGACGGCTCC
841      TGGGTCCAGG TCGCAGAGAA GAGGGCAGTC CTGGCTCATG TTGATGTGCA GACTCTGTCC
901      AGCCAGCTGG CGGTGACCGT GGGACCTGGT GAACGTCGGA TTGGCCCAGG CGAACCCTTA
961      GAACTGCTGT GCAATGTGTC CGGTGCACTG CCCCCACCAG GCCGTCATGC TGCGTACTCT
1021     GTGGGCTGGG AGATGGCCCC TGCAGGGGCT CCTGGACCCG GCCGCCTGGT GGCCCAGCTG
1081     GACACGGAAG GTATAGGCAG CCTGGGCCCT GGCTATGAGG ACCGGCACAT TGCCATAGAG
1141     AAAGTAGCAT CCAGAACCTA CCGACTGCGG CTGGAGGCTG CCAGGCCCGC TGATGCAGGC
1201     ACCTACCGCT GCCTCGCCAA AGCCTATGTT CGAGGGTCTG GGACCCGGCT TCGTGAAGCG
1261     GCCAGTGCTC GTTCCCGGCC TCTCCCTGTG CATGTGAGAG AAGAAGGCGT GGTGCTAGAG
1321     GCTGTGGCGT GGCTAGCAGG GGGCACTGTG TACCGGGGAG AAACGGCCTC CCTGCTATGC
1381     AACATATCTG TGCGGGGCGG CCCCCCAGGG CTGCGACTAG CAGCCAGCTG GTGGGTGGAG
1441     AGGCCAGAGG AGGGCGAGCT GAGCACTGGC CCTGCTCAGC TTGTGGGTGG AGTGGGTCAG
1501     GACGGTGTGG CAGAGCTGGG AGTCCGGCCT GGAGGGGGTC CTGTCAGTGT GGAGCTGGTG
1561     GGACCCAGAA GTCATCGGCT AAGACTGCAC GGCTTGGGGC CTGAGGATGA AGGCATATAC
1621     CACTGTGCCC CAAGTGCCTG GGTGCAGCAT GCGGACTACA GCTGGTACCA GGCAGGCAGT
1681     GCGCGCTCCG GGCCTGTCAC AGTCTACCCC TACACGCATG CTGTGGATAC CCTATTTGTG
1741     CCCCTGTTGG TGGGTACAGG AGTCGCCCTA GTTACCGGCG CCAGCGTCCT GGCTACCATC
1801     ACCTGCTGCT TTATGAAGAG GATGCGGAAG CGGTGA

 

Quality Control:cDNA sequencing results is available upon request.