Anti-IGSF3 Antibody

Cat: 11290-RP02
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Anti-IGSF3 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-IGSF3 Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human IGSF3
Immunogen
Recombinant Human IGSF3 protein (Catalog#11290-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human IGSF3 (rh IGSF3; Catalog#11290-H08H; O75054-1; Met 1-Ala 1124). IGSF3 specific IgG was purified by Human IGSF3 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IGSF3 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 0.5-1 μg/mL  
IHC-P 0.2-2 μg/mL  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IGSF3 Antibody (Rabbit Polyclonal antibody) Images
Human IGSF3 Immunohistochemistry(IHC) 4017
Immunochemical staining of human IGSF3 in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Human IGSF3 Immunohistochemistry(IHC) 4020
Immunochemical staining of human IGSF3 in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Anti-IGSF3 Antibody Alternative Names
Anti-EWI-3 Antibody;Anti-LCDD Antibody;Anti-V8 Antibody
IGSF3 Background Information

Immunoglobulin superfamily member 3 (IGSF3), also known as Glu-Trp-Ile EWI motif-containing protein 3, IgSF3 and EWI-3 and IGSF3, is a single-pass type I membrane protein which belongs to the EWI subfamily of the Ig Superfamily of molecules. IGSF3 has an overall structural similarity and strong sequence similarity to V7, a human leukocyte surface protein. IGSF3 contains eight Ig-like C2-type (immunoglobulin-like) domains. IGSF3 is expressed in a wide range of tissues with high expression in Placenta, kidney and lung. EWI-2 is part of a novel Ig subfamily that includes EWI-F (F2alpha receptor regulatory protein (FPRP), CD9P-1 ), EWI-3 (IGSF3), and EWI-101 (CD101). All four members of this Ig subfamily contain a Glu-Trp-Ile (EWI) motif not seen in other Ig proteins. Human IGSF3 shares 92 % aa identity with mouse IGSF3.

Full Name
immunoglobulin superfamily, member 3
References
  • Saupe S, et al. (1998) Molecular cloning of a human cDNA IGSF3 encoding an immunoglobulin-like membrane protein: expression and mapping to chromosome band 1p13. Genomics. 52(3): 305-11.
  • Lazaar AL, et al. (2001) VCAM-1 activates phosphatidylinositol 3-kinase and induces p120Cbl phosphorylation in human airway smooth muscle cells. J Immunol. 166(1): 155-61.
  • Wetzel A, et al. (2004) Human Thy-1 (CD90) on activated endothelial cells is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18). J Immunol. 172(6): 3850-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IGSF3 Antibody Rabbit Polyclonal ELISA,IHC-P 11290-T24
    Anti-IGSF3 Antibody (APC) Mouse Monoclonal FCM 11290-MM05-A
    Anti-IGSF3 Antibody (FITC) Mouse Monoclonal FCM 11290-MM05-F
    Anti-IGSF3 Antibody Rabbit Polyclonal IHC-P 11290-T08
    Anti-IGSF3 Antibody (PE) Mouse Monoclonal FCM 11290-MM05-P
    Anti-IGSF3 Antibody Rabbit Polyclonal ELISA 11290-RP01
    Anti-IGSF3 Antibody Mouse Monoclonal FCM 11290-MM05
    Anti-IGSF3 Antibody Rabbit Monoclonal ELISA 11290-R002
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