Anti-IGSF3 Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Human
Recombinant Human IGSF3 protein (Catalog#11290-H08H)
Produced in rabbits immunized with purified, recombinant Human IGSF3 (rh IGSF3; Catalog#11290-H08H; O75054-1; Met 1-Ala 1124). IGSF3 specific IgG was purified by Human IGSF3 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IGSF3 Antibody (Rabbit Polyclonal antibody) Validated Applications
|| 0.5-1 μg/mL
|| 0.2-2 μg/mL
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-IGSF3 Antibody (Rabbit Polyclonal antibody) Images
Immunochemical staining of human IGSF3 in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Immunochemical staining of human IGSF3 in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Anti-IGSF3 Antibody Alternative Names
Anti-EWI-3 Antibody;Anti-LCDD Antibody;Anti-V8 Antibody
IGSF3 Background Information
Immunoglobulin superfamily member 3 (IGSF3), also known as Glu-Trp-Ile EWI motif-containing protein 3, IgSF3 and EWI-3 and IGSF3, is a single-pass type I membrane protein which belongs to the EWI subfamily of the Ig Superfamily of molecules. IGSF3 has an overall structural similarity and strong sequence similarity to V7, a human leukocyte surface protein. IGSF3 contains eight Ig-like C2-type (immunoglobulin-like) domains. IGSF3 is expressed in a wide range of tissues with high expression in Placenta, kidney and lung. EWI-2 is part of a novel Ig subfamily that includes EWI-F (F2alpha receptor regulatory protein (FPRP), CD9P-1 ), EWI-3 (IGSF3), and EWI-101 (CD101). All four members of this Ig subfamily contain a Glu-Trp-Ile (EWI) motif not seen in other Ig proteins. Human IGSF3 shares 92 % aa identity with mouse IGSF3.
immunoglobulin superfamily, member 3
Saupe S, et al. (1998) Molecular cloning of a human cDNA IGSF3 encoding an immunoglobulin-like membrane protein: expression and mapping to chromosome band 1p13. Genomics. 52(3): 305-11.Lazaar AL, et al. (2001) VCAM-1 activates phosphatidylinositol 3-kinase and induces p120Cbl phosphorylation in human airway smooth muscle cells. J Immunol. 166(1): 155-61.Wetzel A, et al. (2004) Human Thy-1 (CD90) on activated endothelial cells is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18). J Immunol. 172(6): 3850-9.