Human IGHA1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13561-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IGHA1 cDNA Clone Datasheet

IGHA1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC016369.1
cDNA Size:	1062
Gene Synonym:	IgA1
Source:	Human

IGHA1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens immunoglobulin heavy constant alpha 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 759 C/T and 777 C/T not causing the amino acid variation.

IGHA1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IGHA1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IGHA1 cDNA Clone Sequence and Quality Control

Sequence:

1        GCATCCCCGA CCAGCCCCAA GGTCTTCCCG CTGAGCCTCT GCAGCACCCA GCCAGATGGG
61       AACGTGGTCA TCGCCTGCCT GGTCCAGGGC TTCTTCCCCC AGGAGCCACT CAGTGTGACC
121      TGGAGCGAAA GCGGACAGGG CGTGACCGCC AGAAACTTCC CACCCAGCCA GGATGCCTCC
181      GGGGACCTGT ACACCACGAG CAGCCAGCTG ACCCTGCCGG CCACACAGTG CCTAGCCGGC
241      AAGTCCGTGA CATGCCACGT GAAGCACTAC ACGAATCCCA GCCAGGATGT GACTGTGCCC
301      TGCCCAGTTC CCTCAACTCC ACCTACCCCA TCTCCCTCAA CTCCACCTAC CCCATCTCCC
361      TCATGCTGCC ACCCCCGACT GTCACTGCAC CGACCGGCCC TCGAGGACCT GCTCTTAGGT
421      TCAGAAGCGA ACCTCACGTG CACACTGACC GGCCTGAGAG ATGCCTCAGG TGTCACCTTC
481      ACCTGGACGC CCTCAAGTGG GAAGAGCGCT GTTCAAGGAC CACCTGAGCG TGACCTCTGT
541      GGCTGCTACA GCGTGTCCAG TGTCCTGCCG GGCTGTGCCG AGCCATGGAA CCATGGGAAG
601      ACCTTCACTT GCACTGCTGC CTACCCCGAG TCCAAGACCC CGCTAACCGC CACCCTCTCA
661      AAATCCGGAA ACACATTCCG GCCCGAGGTC CACCTGCTGC CGCCGCCGTC GGAGGAGCTG
721      GCCCTGAACG AGCTGGTGAC GCTGACGTGC CTGGCACGTG GCTTCAGCCC CAAGGATGTG
781      CTGGTTCGCT GGCTGCAGGG GTCACAGGAG CTGCCCCGCG AGAAGTACCT GACTTGGGCA
841      TCCCGGCAGG AGCCCAGCCA GGGCACCACC ACCTTCGCTG TGACCAGCAT ACTGCGCGTG
901      GCAGCCGAGG ACTGGAAGAA GGGGGACACC TTCTCCTGCA TGGTGGGCCA CGAGGCCCTG
961      CCGCTGGCCT TCACACAGAA GACCATCGAC CGCTTGGCGG GTAAACCCAC CCATGTCAAT
1021     GTGTCTGTTG TCATGGCGGA GGTGGACGGC ACCTGCTACT GA

Quality Control:cDNA sequencing results is available upon request.