IGFBP7 / IBP-7 Protein (His Tag)

Insulin-like growth factor binding protein 7 Protein Datasheet

IGFBP7 / IBP-7 Protein Product Information

• Synonym: AGM, FSTL2, IBP-7, IGFBP-7, IGFBP-7v, IGFBPRP1, MAC25, PSF, TAF
• Protein Construction: A DNA sequence encoding the human IGFBP7 (Q16270) (Asp 30- Leu 282) was fused with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Human Cells

IGFBP7 / IBP-7 Protein QC Testing

• Purity: >92 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its ability to bind biotinylated recombinant human CCL21 in a functional ELISA.
  2. Measured by its ability to bind biotinylated recombinant human VEGF165 in a functional ELISA.
  3. Measured by its ability to bind biotinylated recombinant human IGF2 in a functional ELISA.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 27
• Molecular Mass: The secreted recombinant human IGFBP7 comprises 273 amino acids and has a predicted molecular mass of 28.6 kDa. ?The apparent molecular mass of rhIGFBP7 is approximately 36 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IGFBP7 / IBP-7 protein

IGFBP7 / IBP-7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IGFBP7 / IBP-7 Protein Description

Insulin-like growth factor-binding protein 7, also known as IGF-binding protein 7, IBP-7, IGFBP-7, IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, Prostacyclin-stimulating factor, Tumor-derived adhesion factor TAF and IGFBP7, is a secreted protein involved in several cellular processes, including proliferation, senescence and apoptosis. Loss of IGFBP7 expression is a critical step in the development of human tumors, including melanoma and colon cancer. IGFBP7 contains one Ig-like C2-type (immunoglobulin-like) domain, one IGFBP N-terminal domain and one Kazal-like domain. IGFBP7 binds IGF-I and IGF-II with a relatively low affinity. IGFBP7 stimulates prostacyclin (PGI2) production. It also stimulates cell adhesion. The major function of the IGFBP7 protein is in the regulation of availability of Insulin like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. IGFBP7 protein is implicated in some cancers.

References

1. Akaogi K. et al.,1994, Biochem Biophys Res Commun. 198: 1046-53.
2. Oh Y. et al., 1996, J Biol Chem. 271: 30322-30325.
3. Wilson EM. et al., 2001, J Clin Endocrinol Metab. 86: 4504-4511.
4. Sanjida Ahmed., et al., 2006, FEBS Journal.273 (3): 615-27.
5. Gommans WM., et al., 2008, RNA. 14: 2074-2085.
6. Vizioli MG. et al., 2010, Oncogene. 29 (26): 3835-44.