Cynomolgus IGFBP4 Protein (His Tag)

Insulin-like growth factor binding protein 4 Protein Datasheet

IGFBP4 Protein Product Information

• Synonym: IGFBP4
• Protein Construction: A DNA sequence encoding the cynomolgus IGFBP4(Met1-Glu258 ) was expressed with a polyhistidine tag at the C-terminus.
• Source: Cynomolgus
• Expression Host: Human Cells

IGFBP4 Protein QC Testing

• Purity: > 80 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 22
• Molecular Mass: The recombinant heterodimer of cynomolgus IGFBP4 comprises 248 amino acids and has a calculated molecular mass of 27.4 KDa. The apparent molecular mass of the protein is approximately 33-36 KDa respectively in SDS-PAGE.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IGFBP4 protein

IGFBP4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IGFBP4 Protein Description

Insulin-like growth factor-binding proteins (IGFBPs) control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF complexes. Six high-affinity IGF-binding proteins (IGFBP-1 to -6) have been identified, and IGFBP4, the smallest IGFBP expressed by a wide range of cell types, exists in both non-glycosylated and N-glycosylated forms in all biological fluids. Structurally consisting of an IGFBP domain and a thyroglobulin type-I domain, IGFBP4 binds IGF-I and IGF-II with similar affinities as a transport protein and modulates their biological effects. Increasing evidences indicate that IGFBP4 inhibits IGF-induced cellular growth both in vitro and in vivo. As an important member of the IGF system, IGFBP4 is involved in a wide range of biological processes including reproductive physiology, bone formation, renal pathophysiology and carcinomas.

References

1. Durai R. et al., 2006, Int J Oncol. 28: 1317-25.
2. Sitar T. et al., 2006, Proc Natl Acad Sci. 103: 13028-33.
3. Zhou R. et al., 2003, J Endocrinol. 178: 177-93.
4. Zhou J. et al., 2003, Biol Reprod. 69: 22-9.