Cynomolgus monkey IGFBP2 Gene cDNA Clone / ORF Clone

IGFBP2 cDNA Clone Datasheet

IGFBP2 cDNA Clone Product Information

• cDNA Size: 981
• Gene Synonym: IGFBP2
• Source: Cynomolgus monkey

IGFBP2 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) insulin-like growth factor binding protein 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 953 A/G resulting in the amino acid His substitution by Arg.
  Please check the sequence information before order.

IGFBP2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IGFBP2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IGFBP2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCCGA GAGTGGGCTG CCCTGCGCTG CCGCTGCCGC CGCCGCCGCT GCTGCCGCTG
61       CTGCTGCTTC TGCTGGGCGC GAGTGGCGGC GGCGGCGGCG GGGCGCACGC GGAGGTGCTG
121      TTCCGCTGCC CTCCCTGCAC ACCCGAGCGC CTGGCCGCCT GCGGGCCCCC GCCGGTCGCG
181      CCGCCGGCCG CGGTGGCCGC GGTGGCCGGA GGAGCCCGCA TGCCATGCGC CGAGCTAGTC
241      CGGGAGCCGG GCTGCGGCTG CTGCTCGGTG TGCGCCCGGC TGGAGGGCGA GGCGTGCGGC
301      GTCTACACCC CGCGCTGCGG CCAGGGGCTG CGCTGCTATC CCCACCCGGG CTCCGAGCTG
361      CCCCTGCAGG CGCTGGTTAT GGGCGAGGGC ACTTGTGAGA AGCGCCGGGA CGCCGAGTAT
421      GGCGCCAGCC CGGAGCAGGT TGCAGACAAT GGCGATGACC ACTCGGAAGG AGGCCTGGTG
481      GAGAACCACG TGGACAGCAC CATGAACATG TTGGGCGGGG GAGGCAGTGC AGGCCGGAAG
541      CCCCTCAAGT CGGGTATGAA GGAGCTGGCT GTGTTCCGGG AGAAGGTCGC TGAGCAGCAC
601      CGGCAGATGG GCAAGGGTGG CAAACATCAC CTCGGCCTGG AGGAGCCCAA GAAGCTGCGA
661      CCACCCCCTG CGAGGACTCC CTGCCAGCAG GAACTGGACC AGGTCCTGGA GCGGATCTCC
721      ACCATGCGCC TTCCGGATGA GCGGGGCCCT CTGGAGCACC TCTACTCCCT GCACATCCCC
781      AACTGTGACA AGCATGGCCT GTACAACCTC AAACAGTGCA AGATGTCTCT GAACGGGCAG
841      CGTGGGGAGT GCTGGTGTGT GAACCCCAAC ACCGGGAAGC TGATCCAGGG AGCCCCCACC
901      ATCCGGGGGG ACCCTGAGTG TCATCTCTTC TACAATGAGC AGCAGGAGGC CCGCGGGGTG
961      CACACCCAGC GGATGCAGTA G

 

Quality Control:cDNA sequencing results is available upon request.