Mouse IGF1R Gene cDNA Clone / ORF Clone

IGF1R cDNA Clone Datasheet

IGF1R cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010513.2
• cDNA Size: 4110
• Gene Synonym: hyft; CD221; IGF-1R; D930020L01; A330103N21Rik; Igf1r
• Source: Mouse

IGF1R cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus insulin-like growth factor I receptor DNA.
• Vector: Vector 3. Restriction site: HindIII + XbaI
• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 3878-3890 deletion mutation

IGF1R cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IGF1R cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IGF1R cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGTCTG GCTCCGGAGG AGGGTCCCCG ACCTCGCTGT GGGGGCTCGT GTTTCTCTCC
61       GCCGCGCTCT CTCTCTGGCC GACGAGTGGA GAAATCTGTG GGCCCGGCAT TGACATCCGC
121      AACGACTATC AGCAGCTGAA GCGCCTGGAA AACTGCACGG TGATCGAGGG CTTCCTCCAC
181      ATCCTGCTCA TCTCCAAGGC CGAGGACTAC CGAAGCTACC GCTTCCCCAA GCTCACCGTC
241      ATCACTGAGT ACTTGCTGCT CTTCCGAGTC GCTGGCCTCG AGAGCCTGGG AGACCTCTTC
301      CCCAACCTCA CAGTCATCCG TGGCTGGAAA CTCTTCTACA ACTACGCACT GGTCATCTTC
361      GAGATGACCA ATCTCAAGGA TATTGGGCTT TATAATCTGA GGAACATTAC TCGGGGGGCC
421      ATCAGGATTG AGAAGAACGC CGACCTCTGT TACCTCTCCA CCATAGACTG GTCTCTCATC
481      TTGGATGCGG TGTCCAATAA CTACATTGTG GGGAACAAGC CCCCGAAGGA ATGTGGGGAC
541      CTGTGTCCAG GGACATTGGA GGAGAAGCCC ATGTGTGAGA AGACCACCAT CAACAATGAG
601      TACAACTACC GCTGCTGGAC CACAAATCGC TGCCAGAAAA TGTGCCCAAG TGTGTGCGGG
661      AAGCGAGCCT GCACCGAGAA CAACGAGTGC TGCCACCCGG AGTGCCTGGG CAGCTGCCAC
721      ACACCGGACG ACAACACAAC CTGCGTGGCC TGCAGACACT ACTACTACAA AGGCGTGTGT
781      GTGCCTGCCT GCCCGCCTGG CACCTACAGG TTCGAGGGCT GGCGCTGTGT GGATCGCGAT
841      TTCTGCGCCA ACATCCCCAA CGCTGAGAGC AGTGACTCGG ATGGCTTCGT TATCCACGAC
901      GATGAGTGCA TGCAGGAGTG TCCCTCAGGC TTCATCCGCA ACAGCACCCA GAGCATGTAC
961      TGTATCCCCT GCGAAGGCCC CTGCCCCAAA GTCTGCGGCG ATGAAGAGAA GAAAACGAAA
1021     ACCATCGATT CGGTGACTTC TGCTCAAATG CTCCAAGGAT GCACCATCCT GAAGGGCAAT
1081     CTGCTTATTA ACATCCGGAG AGGCAATAAC ATTGCCTCGG AGTTGGAGAA CTTCATGGGG
1141     CTCATCGAGG TGGTGACCGG CTACGTGAAG ATCCGCCATT CTCATGCCTT GGTCTCCTTG
1201     TCCTTCCTGA AGAACCTTCG TCTCATCTTA GGAGAGGAGC AGCTGGAAGG GAACTACTCC
1261     TTCTATGTCC TAGACAACCA GAACTTGCAG CAGCTGTGGG ACTGGAACCA CCGGAACCTG
1321     ACCGTCAGGT CCGGAAAGAT GTACTTTGCT TTCAATCCCA AGCTGTGTGT CTCCGAAATT
1381     TACCGCATGG AGGAAGTGAC CGGAACCAAG GGACGCCAGA GCAAAGGGGA CATAAACACC
1441     AGGAACAACG GAGAGCGAGC TTCCTGTGAA AGTGATGTTC TCCGTTTCAC CTCCACCACG
1501     ACCTGGAAGA ACCGAATCAT CATAACGTGG CACCGGTACC GGCCGCCGGA CTACCGGGAT
1561     CTCATCAGCT TCACAGTTTA CTACAAGGAG GCACCATTTA AAAACGTTAC GGAATATGAC
1621     GGGCAGGATG CCTGTGGCTC CAACAGCTGG AACATGGTGG ATGTAGACCT GCCTCCGAAC
1681     AAGGAGGGCG AGCCTGGCAT TTTACTGCAT GGGCTGAAGC CCTGGACCCA GTATGCTGTC
1741     TATGTCAAGG CTGTGACCCT CACCATGGTG GAAAACGACC ATATCCGTGG GGCCAAAAGT
1801     GAAATCTTGT ACATTCGCAC CAATGCTTCA GTCCCTTCCA TTCCCCTAGA TGTCCTCTCA
1861     GCATCAAACT CTTCCTCTCA GCTGATTGTG AAGTGGAATC CTCCAACTCT GCCCAATGGT
1921     AACTTGAGTT ACTACATTGT GAGGTGGCAG CGGCAGCCCC AGGATGGTTA CCTGTACCGG
1981     CACAACTACT GCTCCAAAGA CAAAATACCC ATCAGAAAGT ACGCCGATGG TACCATCGAC
2041     GTGGAGGAGG TGACGGAAAA TCCCAAGACA GAAGTGTGTG GTGGTGATAA AGGGCCATGC
2101     TGCGCTTGCC CTAAAACTGA AGCTGAGAAG CAGGCTGAGA AGGAGGAGGC TGAGTACCGT
2161     AAAGTCTTTG AGAATTTCCT TCACAATTCC ATCTTTGTGC CCAGGCCCGA AAGGAGGCGG
2221     AGAGACGTCA TGCAAGTGGC CAACACGACC ATGTCCAGCC GAAGCAGGAA CACCACGGTA
2281     GCTGACACCT ACAATATCAC AGACCCGGAG GAGTTCGAGA CAGAGTACCC TTTCTTTGAG
2341     AGCAGAGTGG ATAACAAGGA GAGGACTGTC ATCTCCAACC TCCGGCCTTT CACTCTGTAC
2401     CGCATCGATA TCCACAGCTG CAACCACGAG GCTGAGAAGC TGGGCTGCAG CGCCTCCAAC
2461     TTCGTCTTTG CGAGAACCAT GCCAGCAGAA GGAGCAGATG ATATCCCTGG TCCGGTGACC
2521     TGGGAGCCAA GACCCGAAAA CTCCATCTTT TTAAAGTGGC CAGAACCCGA GAACCCCAAC
2581     GGATTGATCC TAATGTATGA AATTAAATAC GGGTCGCAAG TCGAGGATCA GCGGGAATGT
2641     GTGTCCAGAC AGGAGTACAG GAAGTACGGA GGGGCCAAAC TCAACCGTCT AAACCCAGGG
2701     AACTATACAG CCCGGATTCA GGCTACCTCC CTCTCTGGGA ATGGGTCATG GACAGATCCT
2761     GTGTTCTTCT ATGTCCCCGC CAAAACGACG TATGAGAACT TCATGCATCT GATCATTGCT
2821     CTGCCGGTTG CCATCCTGCT GATCGTTGGG GGGCTGGTTA TCATGCTGTA TGTCTTCCAT
2881     AGAAAGAGAA ATAACAGCAG GTTGGGCAAT GGAGTGCTGT ATGCTTCTGT GAACCCCGAG
2941     TATTTCAGCG CAGCTGATGT GTACGTGCCT GATGAATGGG AGGTAGCTCG AGAGAAGATC
3001     ACCATGAACC GGGAGCTCGG ACAAGGGTCC TTTGGGATGG TCTATGAAGG AGTGGCCAAG
3061     GGTGTGGTCA AGGATGAACC CGAAACCAGA GTGGCCATCA AGACGGTAAA CGAGGCTGCA
3121     AGTATGCGTG AAAGAATCGA GTTTCTCAAC GAGGCCTCGG TGATGAAGGA GTTCAATTGT
3181     CACCATGTGG TCCGGTTGCT GGGTGTGGTA TCCCAAGGCC AGCCCACCCT GGTCATCATG
3241     GAACTAATGA CACGCGGTGA TCTCAAAAGT TATCTCCGGT CTCTGAGGCC AGAAGTGGAG
3301     CAGAATAATC TAGTCCTCAT TCCTCCGAGC TTAAGCAAGA TGATCCAGAT GGCTGGAGAG
3361     ATTGCAGATG GCATGGCCTA CCTCAATGCC AACAAGTTCG TCCACAGAGA CCTTGCTGCT
3421     AGGAACTGCA TGGTAGCCGA AGATTTCACA GTCAAAATTG GAGATTTCGG TATGACACGA
3481     GACATCTACG AGACGGACTA CTACCGGAAA GGCGGGAAGG GGTTGCTGCC TGTGCGCTGG
3541     ATGTCTCCCG AGTCCCTCAA GGATGGTGTC TTCACTACTC ATTCTGATGT CTGGTCCTTC
3601     GGGGTCGTCC TCTGGGAGAT CGCCACGCTG GCTGAGCAGC CCTACCAGGG CTTGTCCAAC
3661     GAGCAAGTTC TTCGTTTCGT CATGGAGGGT GGCCTTCTGG ACAAGCCGGA CAACTGCCCT
3721     GATATGCTGT TTGAACTTAT GCGCATGTGC TGGCAGTATA ACCCCAAGAT GCGGCCCTCC
3781     TTCCTGGAGA TCATCGGCAG CATCAAGGAT GAGATGGAGC CCAGCTTCCA GGAGGTCTCC
3841     TTCTACTACA GCGAGGAGAA CAAGCCTCCC GAGCCAGAGG AGCCTGAGAA CATGGAGAGC
3901     GTCCCACTGG ACCCTTCGGC CTCCTCAGCC TCCCTGCCTC TGCCTGAAAG ACACTCAGGA
3961     CACAAGGCTG AGAATGGCCC GGGCCCTGGC GTGCTCGTTC TCCGCGCCAG TTTTGATGAG
4021     AGACAGCCTT ACGCTCACAT GAACGGGGGA CGCGCCAACG AGAGGGCCTT GCCTCTGCCC
4081     CAGTCCTCGA CCTGCTGA

 

Quality Control:cDNA sequencing results is available upon request.