IFNW1 Protein (His Tag) (Cytokine)

Interferon, omega 1 Protein Datasheet

IFNW1 Protein Product Information

• Synonym: IFNW1
• Protein Construction: A DNA sequence encoding the human IFNW1 (P05000) (Met1-Ser195) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

IFNW1 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Bio-activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV).
  The ED₅₀ for this effect is typically 2-8 pg/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 22
• Molecular Mass: The recombinant human IFNW1 consists of 185 amino acids and predicts a molecular mass of 21.6 KDa. It migrates as an approximately 22 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IFNW1 protein

IFNW1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFNW1 Protein Description

IFNs are a large family of proteins having antiviral, antiproliferative, and immunomodulatory effects, and are divided into two major classes, type I  and type I I, on the basis of differences in receptor binding and nucleotide sequence. Type I IFNs consist of IFN α, β, τ, and ω and bind to the type I  IFN receptor, whereas IFN-γ is the only type I I IFN and is specific for the type I I IFN receptor. Human IFN-ω, was identified by three independent groups in 1985 and is structurally related to IFN-α and -β. Both human IFN-ω and IFN-α are produced by virally induced leukocytes and have similar antiviral activities on human cell lines, and a sizeable proportion (at least 10%) of the total antiviral activity of leukocyte IFN is contributed by IFN-ωl. In addition, it was reported that IFN-ω could inhibit the growth of human tumors in vivo.

References

1. Holly M Horton. et al., 1999, Cancer Research. 59: 4064-8.
2. Rudolf Hauptmann Peter Swetly. 1985, Nucleic acids Research. 13 (13): 4739-49.
3. Günther R Adolf. et al., 1990, The Journal of Biological Chemistry. 265 (16): 9290-5.