Rat IFNGR1 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80328-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IFNGR1 cDNA Clone Datasheet

IFNGR1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_053783.1
cDNA Size:	1395
Gene Synonym:	Ifngr; Ifngr1
Source:	Rat

IFNGR1 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus interferon gamma receptor 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 204 A/T, 205 T/A, 1076 T/C resulting in the amino acid glu substitution by asp, tyr substitution by asn, leu substitution by pro and 201A/G，333G/A not causing the amino acid variation.

IFNGR1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IFNGR1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IFNGR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATTCTGC TGGTGGTCCT GATGCTGTCT GCGGAGATCG GGAGTGGAGC TTTGATGAGC
61       ACCGAGGATC CTAAGCCGCC CTCGGTGCCT GCGCCAACAA ATGTTCTAAT TACGTCCTAT
121      GACTTGAACC CTGTCGTACA TTGGAAGCAC CAGAACGTGT CGCAGGCTGC CGTCTTCACT
181      GTACAGGTAA AGATGTATCC GGATAACTGG ACTGATGCCT GCACCAACAT TGCCCATCAT
241      TATTGTAATA TCTACAAACA CATTTCCTAT CCTGACTCAT CTGCCTGGGC CAGAGTTAAG
301      GCCAAGGTTG GACAAAGAGA ATCTGCCTAT GCACAGTCAG AAGAGTTTAT TATGTGCCGA
361      AAGGGGAAGG TTGGACCGCC TGGCCTGGAC ATCGGAAGGA AGGAAGATCA GCTGATTGTC
421      CACATATTTC ACCCTAAGGT CAATGTGAGT CAGGAAACCA TGTTTGGTGA CGGAAATACC
481      TGTTACACAT TCGACTACAC TGTGTTTGTG AAACATTACA GGAGTGGGGA GATCCTACAT
541      ACAGAACATA GCGTCCTAAA AGAAGATTGT AGCGAAACTC TGTGTGAGTT AAACATCTCA
601      GTGTCCACGC TGAATTCCAA TTACTGTGTT TCAGTAGTTG GAAAGTCGTC TTTCTGGCAA
661      GTTAATACAG AAACATCAAA AGACGCCTGT ATCCCCTTTC TCCATGATGA CAGAGAAGAA
721      TCAATTTGGA TGCTGCTTGT TGCTCCTCTT CTCTTCCTTA CAATAGTTGT CCCGGCACTT
781      GTGTGTTGTT ACATTAAGAA GAATCCATTT AAGAGAAAAA GCATAATGTT ACCTAAGTCC
841      TTGCTCTCTG TGGTAAAAAA TGCCACGTCC GAGACCAAAC CTGAATCAAA GTACTCCCTT
901      GTCACGTCGT GCCAGCCAGC TGTCCTAGAG AACGAGACAG TGATCTGTGA GGAGCACTTG
961      TCCACAGTGA CAACTCCAGA CAGCCTAGAA GCACCAGAGC AGGAAGAACT TTCTAAAGGA
1021     ACCGTGGCTA CGGTTGCTGA AGGAAACACT TCCCCCGAGA CCACCGACAG CCCTCCAACT
1081     CCGGTACAAA GTGGCCATTT TTCCCTGTCA AGTAGTAACC AGTCAGGCTC CTGTAGCCTC
1141     ACCACCTATC ACTCCAGGGA CGGCTCTGAC AGTGGCCTCG TGGGCACAGG CAGCTCCATA
1201     TCTGACTCTG ACTTTCTCCC AAACAACGAC TCGGAAACAA AGATGGCAGA CCCAGCCCCT
1261     ACACCTGTGA GGAAGGCCCT GACTTTCTCC GGTTATGACA AACCACACGT GTTGGTGGAC
1321     GTGCCCGTGG ATGGGGAGGG CAAGGAGTCT TTGATTGGGT ATAGACTCAC GGGTGATACC
1381     CAGGAACTGT CCTAA

Quality Control:cDNA sequencing results is available upon request.

IFNGR1/CD119 related areas, pathways, and other information

Cytokine