Mouse ICOS Gene cDNA Clone / ORF Clone

ICOS cDNA Clone Datasheet

ICOS cDNA Clone Product Information

• Gene Bank Ref.ID: NM_017480.2
• cDNA Size: 603
• Gene Synonym: H4; CCLP; AILIM; CRP-1; Ly115
• Source: Mouse

ICOS cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus inducible T-cell co-stimulator DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

ICOS cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ICOS cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ICOS cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGCCGT ACTTCTGCCG TGTCTTTGTC TTCTGCTTCC TAATCAGACT TTTAACAGGA
61       GAAATCAATG GCTCGGCCGA TCATAGGATG TTTTCATTTC ACAATGGAGG TGTACAGATT
121      TCTTGTAAAT ACCCTGAGAC TGTCCAGCAG TTAAAAATGC GATTGTTCAG AGAGAGAGAA
181      GTCCTCTGCG AACTCACCAA GACCAAGGGA AGCGGAAATG CGGTGTCCAT CAAGAATCCA
241      ATGCTCTGTC TATATCATCT GTCAAACAAC AGCGTCTCTT TTTTCCTAAA CAACCCAGAC
301      AGCTCCCAGG GAAGCTATTA CTTCTGCAGC CTGTCCATTT TTGACCCACC TCCTTTTCAA
361      GAAAGGAACC TTAGTGGAGG ATATTTGCAT ATTTATGAAT CCCAGCTCTG CTGCCAGCTG
421      AAGCTCTGGC TACCCGTAGG GTGTGCAGCT TTCGTTGTGG TACTCCTTTT TGGATGCATA
481      CTTATCATCT GGTTTTCAAA AAAGAAATAC GGATCCAGTG TGCATGACCC TAATAGTGAA
541      TACATGTTCA TGGCGGCAGT CAACACAAAC AAAAAGTCTA GACTTGCAGG TGTGACCTCA
601      TAA

 

Quality Control:cDNA sequencing results is available upon request.