Ferret IFNG Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
FG60007-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IFNG cDNA Clone Datasheet

IFNG cDNA Clone Product Information

Gene Bank Ref.ID:	AB300566.1
cDNA Size:	501
Gene Synonym:	IFNG
Source:	Ferret

IFNG cDNA Clone Description

cDNA Description:	ORF Clone of Ferret Interferon gamma DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 472 C/G and 479-480 GA/CC resulting in the amino acid leu substitution by val, arg substitution by pro.

IFNG cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IFNG cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IFNG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATTATA CAACTATATG CTTAGCTTTT CAGCTTTGCG TGATTTTCTG TTCTTCTGGC
61       TATTACTGTC AGGCCATGTT TTTTAAAGAA ATAGAAGACC TAAAGGAATA TTTTAATGCA
121      AGTAATCCAG ATGTAGCAGA TGGTGGGCCT CTTTTCTTAG ATATTTTGAA GAACTGGAGA
181      GAGGAGAGTG ACAAAAAAAT CATTCAAAGC CAAATTGTCT CCTTCTACTT GAAACTGTTT
241      GAAAACTTCA AAGATAACCA GATCATTCAA AGGAGCATGG ATACCATCAA GGAAGACATG
301      CTTGTCAGGT TCTTCAATAA CAGCAGCAGT AAGCTGGAGG ACTTCCTTAA GCTGATTCGA
361      ATTCCTGTGA ATGATCTGCA GGTCCAGCGC AAAGCGATAA ATGAACTCAT CAAAGTGATG
421      AATGATCTCT CACCAAGATC TAACCTAAGG AAGCGGAAAA GGAGTCACAG TGTGTTTCCC
481      GGCCGCAGAG CATCGAAATA A

Quality Control:cDNA sequencing results is available upon request.

IFNG related areas, pathways, and other information

Cytokine