Rat IFNG Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80234-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IFNG cDNA Clone Datasheet

IFNG cDNA Clone Product Information

Gene Bank Ref.ID:	NM_138880.2
cDNA Size:	471
Gene Synonym:	IFNG2; Ifng
Source:	Rat

IFNG cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus interferon gamma DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

IFNG cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IFNG cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IFNG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGTGCTA CACGCCGCGT CTTGGTTTTG CAGCTCTGCC TCATGGCCCT CTCTGGCTGT
61       TACTGCCAAG GCACACTCAT TGAAAGCCTA GAAAGTCTGA AGAACTATTT TAACTCAAGT
121      AGCATGGATG CTATGGAAGG AAAGAGCCTC CTCTTGGATA TCTGGAGGAA CTGGCAAAAG
181      GACGGTAACA CGAAAATACT TGAGAGCCAG ATTATCTCTT TCTACCTCAG ACTCTTTGAA
241      GTCTTGAAAG ACAACCAGGC CATCAGCAAC AACATAAGTG TCATCGAATC GCACCTGATC
301      ACTAACTTCT TCAGCAACAG TAAAGCAAAA AAGGATGCAT TCATGAGCAT CGCCAAGTTC
361      GAGGTGAACA ACCCACAGAT CCAGCACAAA GCTGTCAATG AACTCATCAG AGTGATTCAC
421      CAGCTGTCAC CAGAATCTAG CCTAAGGAAG CGGAAAAGGA GTCGGTGCTG A

Quality Control:cDNA sequencing results is available upon request.

IFNG related areas, pathways, and other information

Cytokine