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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat IFNG ORF mammalian expression plasmid, C-GFPSpark tag||RG80234-ACG|
|Rat IFNG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80234-ACR|
|Rat IFNG ORF mammalian expression plasmid, C-Flag tag||RG80234-CF|
|Rat IFNG ORF mammalian expression plasmid, C-His tag||RG80234-CH|
|Rat IFNG ORF mammalian expression plasmid, C-Myc tag||RG80234-CM|
|Rat IFNG ORF mammalian expression plasmid, C-HA tag||RG80234-CY|
|Rat IFNG ORF mammalian expression plasmid, N-Flag tag||RG80234-NF|
|Rat IFNG ORF mammalian expression plasmid, N-His tag||RG80234-NH|
|Rat IFNG ORF mammalian expression plasmid, N-Myc tag||RG80234-NM|
|Rat IFNG ORF mammalian expression plasmid, N-HA tag||RG80234-NY|
|Rat IFNG natural ORF mammalian expression plasmid||RG80234-UT|
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IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.