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Cynomolgus monkey IFNB1 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog Vector (Price) Quantity In Stock Operation
CG90104-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IFNB1 cDNA Clone Datasheet

 

IFNB1 cDNA Clone Product Information

cDNA Size:

564

Gene Synonym:

IFNB1

Source: Cynomolgus monkey

IFNB1 cDNA Clone Description

cDNA Description:

ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) interferon, beta 1, fibroblast DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 322 T/C,412 A/G,resulting in the amino acid Phe substitution by Leu,Met substitution by Val and 153 C/T not causing the amino acid variation.
Please check the sequence information before order.

IFNB1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IFNB1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IFNB1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCAACA AGTGTCTCCT CCAAATTGCT CTCCTGTTGT GCTTCTCCAC TACGGCTCTT
61       TCCATGAGCT ACAACTTGCT TGGATTCCTA CAAAGAAGCA GCAGTTTTCA GTGTCAGAAG
121      CTCCTGTGGC AATTGAATGG AAGGCTTGAA TATTGCCTCA AGGACAGGAT GAACTTTGAC
181      ATCCCTGAGG AAATTAAGCA GCCGCAGCAG TTCCAGAAGG AGGACGCTGC ATTGACCATC
241      TATGAGATGC TCCAGAACAT CTTCGCTATT TTCAGACAAG ATTTATCTAG CACTGGCTGG
301      AATGAGACTA TTGTGGAGAA CCTCCTTGCT AATGTCTATC ATCAGATAGA CCATCTGAAG
361      ACAATCCTAG AAGAAAAACT GGAGAAAGAA GATTTCACCA GGGGAAAATT CGTGAGCAGT
421      TTGCACCTGA AAAGATACTA TGGAAGGATT CTGCATTACC TGAAGGCCAA GGAGTACAGT
481      CACTGTGCCT GGACCATAGT CAGAGTGGAA ATCCTCAGGA ACTTTTTCTT CATTAACAAA
541      CTTACAGGTT ACCTCCGAAA CTGA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

IFNB1 (IFN-beta/Interferon beta) related areas, pathways, and other information