Mouse IFNB1 / IFN-beta / Interferon beta Protein (Cytokine)

Interferon beta 1, fibroblast Protein Datasheet

IFNB1 / IFB / Fibroblaset / IFNb Protein Product Information

• Synonym: RP23-19G2.2, IFN-beta, IFNB, Ifb
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse IFNB1 (P01575) (Met 1-Asn 182) was fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

IFNB1 / IFB / Fibroblaset / IFNb Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ile 22
• Molecular Mass: The secreted recombinant mouse IFNB1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 402 amino acids and has a calculated molecular mass of 46.7 kDa. As a result of glycosylation, the apparent molecular mass of rmIFNB1/Fc monomer is approximately 58 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IFNB1 / IFB / Fibroblaset / IFNb protein

IFNB1 / IFB / Fibroblaset / IFNb Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFNB1 / IFB / Fibroblaset / IFNb Protein Description

Interferon beta 1(IFN beta1) or IFNB1 is one type of IFN beta proteins that is a member of the Interferon type I family. Interferon beta 1(IFN beta1) or IFNB1 is produced in large quantities by fibroblasts and binds to a specific cell surface receptor complex known as the IFN alpha receptor (IFNAR) that consists of IFNAR1 and IFNAR2 chains as other members of IFN1 famlily do. The induction of interferon beta (IFNB1) is a key event in the antiviral immune response. It has shown that treatment with IFN beta affects NK cell cycle without altering NK cell apoptosis in MS patients. Treated with IFN beta alone or in combination with low-dose prednisolone displayed increased proportion of all NK cell subsets in the active phase of the cell cycle. There was no difference in NK cell apoptosis markers. Synthetic interferons (IFNs) are used in the treatment of several types of cancer. It has also been proved that apoptosis was decreased by IFN treatment, whereas inhibition of proliferation is caused by cell cycle arrest in late S-phase.

References

1. Sanvito L, et al. (2011) J Neuroimmunol. 236 (1-2) : 111-7.
2. Erdmann J, et al. (2011) J Interferon Cytokine Res. 31 (5) : 451-8. 
3. Biron CA, et al. (2002) Curr Top Microbiol Immunol. 263: 7-27.