Cynomolgus monkey IFNAR2 Gene cDNA Clone / ORF Clone (Cytokine)

IFNAR2 cDNA Clone Datasheet

IFNAR2 cDNA Clone Product Information

• cDNA Size: 1551
• Gene Synonym: IFNAR2
• Source: Cynomolgus monkey

IFNAR2 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) interferon (alpha, beta and omega) receptor 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 305 C/T, 1196 C/A, 1223 A/G, 1432 A/G, 1503 G/C resulting in the amino acid Thr substitution by Ile, Ala substitution by Glu, Glu substitution by Gly, Thr substitution by Ala, Glu substitution by Asp and 672 C/T, 762 G/A not causing the amino acid variation.
  Please check the sequence information before order.

IFNAR2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IFNAR2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IFNAR2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTTTTGA GCCAGAATGC CTTCATCGTC AGACCGCTTA ATTTGTTTCT CATGGTGTGT
61       ATCAGCCTCG TGTTTGGTAT TTCACATGAT TTGCCTGATT ACACAAGTGA ATCTTGCACT
121      TTTAAGATCT CATTGCGAAA TTTCCGGTCC ATCTTATCAT GGGAATTAAA AAACCACTCC
181      ATTGTGGCAA CTCACTATAA GTTGCTGTAT ACAATCATGA GTAAACCAGA AGATTTGAAG
241      ATAGTTAAGA ACTGTGCAAA TACCACAAGA TCATTTTGTG ACCTCACAGA TGAGTGGAGA
301      AGCATACACG AGGCCTATGT CACCAGCCTA GAAGGATTCA GCGGGAACAC AACCTTGTTC
361      AATTGCTCAC ACAATTTCTG GCTGGACATA GACATGTCTT TTGAACCGCC AGAGTTTGAG
421      ATTGTTGGTT TTACCAACCA CATTAATGTG ATCGTGAAAT TTCCATCTAT TGTTGAGGAA
481      GAATTACAGT TTGATTTATC ACTTGTCATT GAAGAGCAGT CAGAGGGGAT TGTTAAGAAG
541      CATAAACCCA CAATAAAAGG AAACATGAGT GGAAATTTCA CCTATATCAT TGACAAGTTA
601      ATTCCAAACA CAAACTACTG TGTATCTGTT TATTTCGATC ATAACGATGA GCAGGCAGTC
661      ATAAAGTCTC CTTTAAAATG CACCCTCCTT CAACCTGGCC AGGAATCAGA ATCAGCAGAA
721      TCTGCCAAAG TAGGAGGAAT AATTACTGTG TTTTTGATAG CATTGGTCTT GATAAGCACC
781      ATAGTGACAC TGAAATGGAT TGGTTATATA TGCTTAAGAA ACAGCCTCCC CAAAGTCTTG
841      AATTTTCATA ACTTTTTAGC CTGCCCATTT CCTAACCTGC CACCGTTGGA AGCCATGGAT
901      ATAGTGGAGG TCATTTACAT CAACAGAAAG AAGAAAGTGT GGGATTATAA TTATGATGAT
961      GAAAGTGACA GTGATACTGA GGCAGCACTC AGGACAAGTG GCGGTGGCTA TACCATGCAT
1021     GGACTGACTG TGAGGCCTCT GGGTCAGACC TCCGCCACCT CTACAGAATC CCAGTTTACA
1081     GACCCAGACT CCGAGGAGGA GCCTGACCTG CCTGAGGTTG ATGTGGAGCT CCCCACGACG
1141     CCAAAGTGCA GCCCTCAGCG GTTGGAGCTC TTGAGTGCGC ACTGTGAGAG GAGAGCGAGT
1201     CCGCTCCAGG ACCCCTTTCC CGGAGAGGAC TACAGCTCCA CTGAGGGGTC TGGGGGCAGA
1261     ATTACCTTCA ATGTGGATTT AAACTCTGTA TTTTTGAGGG TTCTTGATGA CGAGGACAGT
1321     GACGACTTAG AAGAAGCTCC TCTGATGGTA TCATCTCATC CGGAAGAGAT GGTTGACCCA
1381     GAAGATCCTG ACAATGTGCA GTCAAGCCAT TTGCTGGCCA GTGGGGAAGG GGCACAGCCA
1441     ACCTTTCCCA GCCCCTCTTC AGAGGGCCTG CAATCTGAAG ATGCTCCATC TGATCAAAGT
1501     GACACTTCTG AGTCAGATGT TGACCTTGGG GATGGTTATA TAATGAGATG A

 

Quality Control:cDNA sequencing results is available upon request.