Anti-IFNAR2 Antibody

Cat: 10359-MM07
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All IFNAR2 Reagents
Anti-IFNAR2 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-IFNAR2 Antibody
Validated applications
ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human IFNAR2
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Immunogen
Recombinant Human IFNAR2 protein (Catalog#10359-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IFNAR2 (rh IFNAR2; Catalog#10359-H08H; NP_997468.1; Met 1-Lys 243). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #07
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IFNAR2 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IFNAR2 Antibody (Mouse Monoclonal antibody) Images
Human IFNAR2/IFNABR Flow Cytometry (FC) 5141
Flow cytometric analysis of human IFNAR2 expression on Jurkat cells. Cells were stained with purified anti-Human IFNAR2, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Anti-IFNAR2 Antibody Alternative Names
Anti-IFN-alpha-REC Antibody;Anti-IFN-R Antibody;Anti-IFNABR Antibody;Anti-IFNARB Antibody
IFNAR2 Background Information

Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at diagnosis assessed by flow cytometry widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNα therapy, IFNAR2 expression at diagnosis was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNα therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNα2, IFNAR2 is internalized, but, instead of being routed towards degradation as it is when complexed to IFNβ, it recycles back to the cell surface.

Full Name
interferon (alpha, beta and omega) receptor 2
References
  • Ito K, et al. (2004) Initial expression of interferon alpha receptor 2 (IFNAR2) on CD34-positive cells and its down-regulation correlate with clinical response to interferon therapy in chronic myelogenous leukemia. Eur J Haematol. 73(3): 191-205.
  • Kim SH, et al. (1997) Mammalian type I interferon receptors consists of two subunits: IFNaR1 and IFNaR2. Gene. 196(1-2): 279-86.
  • Saleh AZ, et al. (2004) Regulated proteolysis of the IFNaR2 subunit of the interferon-alpha receptor. Oncogene. 23(42): 7076-86.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IFNAR2 Antibody Rabbit Polyclonal ELISA 10359-RP01
    Anti-IFNAR2 Antibody Rabbit Polyclonal ELISA 10359-RP02
    Anti-IFNAR2 Antibody Mouse Monoclonal WB 10359-MM03
    Anti-IFNAR2 Antibody (PE) Mouse Monoclonal FCM 10359-MM07-P
    Anti-IFNAR2 Antibody Rabbit Monoclonal WB 10359-R123
    Anti-IFNAR2 Antibody (APC) Mouse Monoclonal FCM 10359-MM07-A
    Anti-IFNAR2 Antibody (FITC) Mouse Monoclonal FCM 10359-MM07-F
    Anti-IFNAR2 Antibody Rabbit Monoclonal ELISA 10359-R117
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