Text Size:AAA

IFNAR2 Antibody (FITC), Mouse MAb

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
IFNAR2Antibody Product Information
Antigen:Recombinant Human IFNAR2 protein (Catalog#10359-H08H)
Clone ID:07
Ig Type:Mouse IgG1
Concentration:10 μl/Test, 0.1 mg/ml
Formulation:Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IFNAR2 (rh IFNAR2; Catalog#10359-H08H; NP_997468.1; Met 1-Lys 243) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
IFNAR2Antibody Usage Guide
Specificity:Human IFNAR2
Application:FCM
Storage:This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Images

Profile of anti-IFNAR2 reactivity on Jurkat cells analyzed by flow cytometry.

Profile of anti-IFNAR2 reactivity on KG-1 cells analyzed by flow cytometry.

Background

Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at diagnosis assessed by flow cytometry widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNα therapy, IFNAR2 expression at diagnosis was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNα therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNα2, IFNAR2 is internalized, but, instead of being routed towards degradation as it is when complexed to IFNβ, it recycles back to the cell surface.

References
  • Ito K, et al. (2004) Initial expression of interferon alpha receptor 2 (IFNAR2) on CD34-positive cells and its down-regulation correlate with clinical response to interferon therapy in chronic myelogenous leukemia. Eur J Haematol. 73(3): 191-205.
  • Kim SH, et al. (1997) Mammalian type I interferon receptors consists of two subunits: IFNaR1 and IFNaR2. Gene. 196(1-2): 279-86.
  • Saleh AZ, et al. (2004) Regulated proteolysis of the IFNaR2 subunit of the interferon-alpha receptor. Oncogene. 23(42): 7076-86.
  • Interferon & Receptor Related Products
    Product nameProduct name
    Cynomolgus / Rhesus IFNG / Interferon Gamma ProteinMouse IFNB1 / IFN-beta / Interferon beta Protein (Fc Tag)Mouse IFNA14 / Interferon alpha-14 Protein (His Tag)Mouse IFNA13 / Interferon alpha-13 Protein (His Tag)Rat IFN-alpha / IFNA1 / IFN Protein (His Tag)Human Interferon beta / IFN-beta / IFNB Protein (Fc Tag)Cynomolgus IFNA13 / Interferon alpha-13 Protein (His Tag)Cynomolgus Interferon alpha-B / IFNA8 Protein (Fc Tag)Cynomolgus Interferon alpha-B / IFNA8 Protein (His Tag)Cynomolgus IFNB1 / IFN-beta / Interferon beta Protein (Fc Tag)Mouse IFNA4 / IFNα4 / Interferon alpha-4 Protein (His Tag)Mouse IL-28B / IFN-lambda-3 Protein (His Tag)Rat IFNA4 / IFNα4 / Interferon alpha-4 Protein (Fc Tag)Human IL-29 / Interleukin-29 Protein (His Tag)Human Interferon alpha 10 / IFNA10 Protein (Fc Tag)Mouse IFNA13 / Interferon alpha-13 Protein (Fc Tag)Mouse IFNB1 / IFN-beta / Interferon beta ProteinMouse IFNA4 / Interferon alpha-4 Protein (Fc Tag)Cynomolgus IFNA2 / Interferon alpha 2 Protein (His Tag)Human Interferon alpha 7 / IFNA7 Protein (Fc Tag)Human IFN omega 1 / IFNW1 Protein (His Tag)Rat IFN-alpha / IFNA1 / IFN Protein (Fc Tag)Rat IFNGR / IFNGR1 Protein (Fc Tag)Human Interferon beta / IFN-beta / IFNB ProteinRat IFNA5 / IFNaG Protein (His Tag)Rat IFNG / Interferon Gamma Protein (Fc Tag)Mouse IFNA5 / IFNaG Protein (His Tag)Human Interferon alpha-B / IFNA8 Protein (His Tag)Mouse IFNGR2 Protein (His Tag)Mouse IFNA14 / Interferon alpha-14 Protein (Fc Tag)Human IL29 / IFNL1 ProteinMouse IFNA5 / IFNaG Protein (Fc Tag)Human IFN-gamma / IFNG / γ-IFN ProteinMouse IFNA2 / Interferon alpha 2 Protein (Fc Tag)Mouse IFNG / Interferon Gamma Protein (Fc Tag)Mouse IFNG / Interferon Gamma Protein (His Tag)Human Interferon omega-1 / IFNω / IFNW1 Protein (Fc Tag)Cynomolgus / Rhesus IFNA14 / Interferon alpha-14 Protein (His Tag)Mouse IFNGR1 / CD119 Protein (Fc Tag)Mouse IFNGR1 / CD119 Protein (His Tag)Human IFNAR1 / IFNAR Protein (Fc Tag)Human IFNAR1 / IFNAR Protein (His Tag)Cynomolgus IFNA2 / Interferon alpha 2 Protein (Fc Tag)Cynomolgus IFNA13 / Interferon alpha-13 Protein (Fc Tag)Cynomolgus IFNAR1 / IFNAR Protein (Fc Tag)Cynomolgus IFNAR1 / IFNAR Protein (His Tag)Cynomolgus IFNAR1 / IFNAR ProteinCynomolgus IFNA4 / IFNα4 / Interferon alpha-4 Protein (Fc Tag)Human Interferon alpha 2 / IFNA2 ProteinCynomolgus IFNGR / IFNGR1 Protein (Fc Tag)Cynomolgus IFNGR / IFNGR1 Protein (His Tag)Mouse IFNG / Interferon Gamma ProteinHuman IFNα4 / IFNa4 / Interferon alpha-4 Protein (Fc Tag)Human IFNα4 / IFNa4 / Interferon alpha-4 Protein (His Tag)Human IFNGR1 / CD119 Protein (His & Fc Tag)Human IFNGR1 / CD119 Protein (His Tag)Human IFNA5 / IFNaG / Interferon alpha-G Protein (Fc Tag)Human Interferon alpha-B / IFNA8 ProteinHuman IFNAR2 / IFNABR Protein (Fc Tag)Human IFNAR2 / IFNABR Protein (His Tag)Human IFNL3 / IL28B / Interleukin-28B Protein (His Tag)Ferret IFNG / Interferon Gamma Protein (His Tag)Mouse IFNAR1 / IFNAR Protein (His Tag)
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"