IFNAR2 Antibody ( FITC ) (Cytokine)

IFNAR2 Antibody Datasheet

IFNAR2 Antibody Product Information

• Immunogen: Recombinant Human IFNAR2 protein (Catalog#10359-H08H)
• Reagents: FITC-conjugated mouse monoclonal antibody
• Clone ID: 07
• Ig Type: Mouse IgG1
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IFNAR2 (rh IFNAR2; Catalog#10359-H08H; NP_997468.1; Met 1-Lys 243) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

IFNAR2 Antibody Usage Guide

• Specificity: Human IFNAR2
• Flow Cytometry:
  

  Profile of anti-IFNAR2 reactivity on Jurkat cells (Left panel) and KG-1 cells (Right panel) analyzed by flow cytometry.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

IFNAR2 Antibody Background

Interferon alpha/beta receptor 2, also known as IFN-alpha/beta receptor 2, Type I interferon receptor 2, IFNAR2 and IFNABR, is a single-pass type I membrane protein which belongs to thetype II cytokine receptor family. IFNAR2 is a receptor for interferons alpha and beta. IFNAR2 was detected in most lymphocytes, monocytes, and granulocytes, although IFNAR2 expression was higher in the monocytes and granulocytes than in the lymphocytes. The intra- and interdaily variations of IFNAR2 in lymphocytes, monocytes, and granulocytes were small. Among the lymphocyte subsets, IFNAR2 showed high expression in natural killer (NK) cells and low expression in T lymphocytes. Isoform 1 and isoform 3 of IFNAR2 are directly involved in signal transduction due to their interaction with the TYR kinase, JAK1. Isoform 1 also interacts with the transcriptional factors, STAT1 and STAT2. Both forms are potent inhibitors of type I IFN activity. Defects in IFNAR2 are associated with susceptibility to hepatitis B virus infection (HBV infection). Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.

References

1. Novick D. et al.,1995, J. Leukoc. Biol. 57: 712-18.
2. Chill JH. et al., 2003, Structure 11: 791-802.
3. Tochizawa,S. et al.,2004, J Pharmacol Toxicol Methods. 50 (1): 59-66.
4. Frodsham A.J. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 9148-53.