Cynomolgus monkey IFNAR1 Gene cDNA Clone / ORF Clone (Cytokine)

IFNAR1 cDNA Clone Datasheet

IFNAR1 cDNA Clone Product Information

• cDNA Size: 1677
• Gene Synonym: IFNAR1
• Source: Cynomolgus monkey

IFNAR1 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) interferon (alpha, beta and omega) receptor 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 853 A/G, 887 A/C, 162 A/T resulting in the amino acid Lys substitution by Glu, Gln substitution by Pro, Glu substitution by Asp and 276 G/C, 357 G/A not causing the amino acid variation.
  Please check the sequence information before order.

IFNAR1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IFNAR1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IFNAR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGGTCA CCCTCCTGGG CGCGACGACC CTAGTGCTGG TCACCTTGGC GCCATGGGTG
61       TTGTCCGCAG CCGCAGGTGG AAAAAATCTA AAATCTCCTC AAAAAGTAGA GGTTGACGTC
121      ATAGATGACA ACTTTATCCT GAGGTGGAAC AGGAGTGAAG ATTCTGTCGG GAATGTGACT
181      TTTTCATTCG ATTATCAAAA ACCTGAGATG GATAATTGGA TAAAATTGCC TGGATGTCAG
241      AATATGACTA GTACCAAATG CAACTTTTCT TCGCTCAAGC TGAACATTTA TGATGAAATT
301      AAATTGCGTA TAAGAGCAGA AAAAGAAAAC ACTTCTTCAT GGTGTGAGGT TGACTCATTT
361      ACACCATTTC GCAAAGCTCA AATTGGTCCT CCAGAAGTAC ATTTAGAAGC TGAAGATAAG
421      GCAATAGTGA TATACATCTC TCCTCCTGGA ACAGAAGATA GTGTCATGTG GGCTTTGGAT
481      CGTTCAAGCT TTACATATAG CTTAGTTATC TGGAAAAACT CTTCAAGTGT AGAAGAAAGG
541      ATTGAAAATA TTTATTCCAG ACATAAAATT TCTAAACTCT CACCAGAGAC TACTTATTGT
601      TTAAAAGTTA AAGCAGCACT ACTTACGTCA CGGAAAATTG GTGTCTATGG TCCAGTGCAT
661      TGTATAAAGA CCACAGTTGA AAATGAACTA CCTCCACCAG AAAATATAGA AGTCATTGTT
721      CAAAATCAGA ACTATGTTCT TAAGTGGGAT TATACGTATG CAAACATGAC CTTTCAAGTT
781      CAGTGGCTCC ATGCCTTTTT AAAAAGGAAA CCTGGAAACC ATTTGTATAA ATGGAAACAA
841      ATACCTGACT GCGAAAATGT CACAACTACC CAGTGTGTCT TTCCTCCAAA CACTTTCCAA
901      AAAGGAATTT ACCTTCTCCG CGTACAAGCA TCTGATGGAA ATAACACGTC TTTTTGGTCT
961      GAAGAGATAA AGTTTGATAC TGAAATACAA GCTTCCCTGC TTCCTCCAGT CTTTAACATT
1021     AGATCCCTTA GTGATTCATT ACATATCTCT ATCGGTGCTC CAAAATGGTC TGAAAACAAG
1081     CCTGTGATCC AGGATTATCC ACTGATTTAT GAAATTCTTT TTTGGGAAAA CACTTCAAAG
1141     GCTGAGAGAA AAATTATCAA GAAAAAAACT GATGTTACTA TTCCTAATTT GAAACCACTG
1201     ACTGTATATT GTGTGAAAGC CAGAGCACAC AGCATGGATG AAAAGCTGAA TAAAAGCAGT
1261     GTTTTTAGTG ATGTTGTGTG TGAGGAAACA AAATCAGGAA ATACATCTAA AATTTGGCTT
1321     ATAATTGGAA TTTTTACTGT ATTACTTGCT CTCCCGTTTG TCATTTATGC TGTGAAAGTC
1381     CTCTTGAGAT GCATCAATTA TGTCTTCTTT CCATCACTTA AACCTTCTTC CAATATAGAT
1441     GAGTATTTCT CTGAACAGTC ATTGAAGAAT CTTCTGCTTT CAACTTCTGA GGAACAAATC
1501     GAAAAATGTC TTATAATTGA AAATATAAGC ACAATTGCTA CAGTAGAAGA AACTAATCAG
1561     ACTGATGAAG ATCATAAAAA ATACAGTTCC CAAACTAGCG AAGATTCAGG AAATTATTCT
1621     AATGAAGATG AAAGTGAAAA TAAAACAAGT GAAGAACTAC AGCAGGACTT TATATGA 

 

Quality Control:cDNA sequencing results is available upon request.