Rat IFNAR1 Gene cDNA Clone / ORF Clone

IFNAR1 cDNA Clone Datasheet

IFNAR1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001105893.1
• cDNA Size: 1320
• Gene Synonym: Ifnar1, Ifnar1_predicted
• Source: Rat

IFNAR1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interferon (alpha, beta and omega) receptor 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 184 T/A resulting in the amino acid ser substitution by thr.

IFNAR1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IFNAR1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IFNAR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGGCAC AGGAGAAACG CTGCTTCAAT TACACGATAC GGATCTGGGG GAGGTCTTTT
61       CCTGTCAGAG AAATTAAACC TATGTATTTT CCAAAAAAGA TATCAAAGCT CTTGCCAGAG
121      ACTACCTACT GTTTAGAAGT CAGAGCAGTG CACACATCGC TTCAGAGACA CAGCAACTAT
181      AGCACAGCGC GGTGTATAAA CACCACAGTG GCAAATAAAA TACCTGTGCC AGAAAATCTA
241      GAAGTGGATG TCCAAGGTGA CAGCTATGTT CTGAAATGGG ACCACGCATT TGCAAACATG
301      ACCTTCAAAG CACAGTGGAT CCCTGTCTAT TCAAAAAGTA GTCCTGGAAA CCATTCAGAC
361      AAATGGAAAC CAATACCTAC CTGTGCAAAT GTGCAGACTA CACACTGTGT ATCTCCTCGA
421      GAGACTTTCC ACACAGGAAC TTTCTTTCTC CGTGTACAAG CCTCAGATGG AAATAACACG
481      TCCTTTTGGT CTGAAGAGAA ACTTATTGAT TCTCAAAAAT ACACTCTCCT TCCTCCTCCG
541      GTCATTGCTG TCACCCCCAC GGGGGACTCC TTGCTTGTTT ATGTCAGCTG TAAGGACAGC
601      AAATGTAATG GACTCATTTA CGAAGTCATC TTTTGGGAAA ACACTTCCAA TACTGAGAAA
661      AGACAACACC CACAAAGCAT GATTAAAGAA CACCCAGAGT TCAACGTTGT GAACCTGCGG
721      CCGCTGACTG TGTACTGTGT CCAGGCTAGA GTGCTCTCCA CTGGCGTGTG GAATAAGACC
781      AGTAAATTCA GTGATAAGCT GTGTAAGAAA ACACATCCAG GAAACTCTTC TGTGACCTGG
841      ATTATAACTG GATTTGGTGC TCTACTCTTC TCTGCCATGG CCTTTTATGC TGGGAGGAGC
901      CTCCAAAAAT ACCTGTATTA CGTGTTCTCC CCACCACTCA AGCCTCCCTG CAGTATCGAC
961      GAGTTTTTCT CAGAGCTGCC TTCCAAAAGC CTTCTGCTTC TGACTGCTGA AGAGCACACC
1021     GAGAGGTGCT TCGTCATTGA GAACACAGGT GTGGTTGCTG GAGAGGAGAA TCGCGCACCT
1081     GAGGAGGAGC TCAGGAAGTA CAGTTCCCAG AGCAGCCAGG ACTCAGGGAA CTACTCCAAC
1141     GAAGAGGAGG AGAGTGTGGG GCCTGAGAGT GGCCAGGATA TGCTTTCCAC AGCTCCCAGT
1201     GGGTCTCCAT GGAGCCTGCC CTGCCCTGCT GGCACCTTGG AAGATGGTGC CTGCTTCCCG
1261     GGAAAAGAAA AGCACCTTCA GAGCCCAGTC CTGAGGACAC AGCCAGCTCT CCACGGCTGA

 

Quality Control:cDNA sequencing results is available upon request.