Mouse IFNAR1 / IFNAR Protein (His Tag)

Mouse Interferon alpha/beta Receptor 1 ( IFNAR1 / IFNAR ) Protein

IFNAR1 / IFNAR Protein Product Information

• Synonym: Ifar, Ifnar, Ifrc, Infar, Ifnar1
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse IFNAR1 ( NP_034638.2 ) extracellular domain ( Met 1 - Thr 429 ) was expressed, with a polyhistidine tag at the C-terminus
• Source: Mouse
• Expression Host: Human Cells

IFNAR1 / IFNAR Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to inhibit rh IFNβ mediated protection of WISH human amnion cells infected with vesicular stomatitis virus ( VSV ) to viral lysis
  The EC₅₀ for this effect is typically 4 - 20 μg/mL
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 27
• Molecular Mass: The recombinant mouse IFNAR1 consists of 414 amino acids and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm IFNAR1 is approximately 55-60 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IFNAR1 protein

IFNAR1 / IFNAR Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFNAR1 / IFNAR Protein Description

Mouse interferon alpha/beta receptor 1, also known as IFN-alpha/beta receptor 1, Type I interferon receptor 1, Cytokine receptor family 2 member 1, Cytokine receptor class-II member 1, CRF2-1, IFNAR1 and IFNAR, is a single-pass type I  membrane protein which belongs to the type I I cytokine receptor family. IFNAR1 is a receptor for interferons alpha and beta which contains three fibronectin type-III domains. The binding of IFNAR1 to type I  IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Ubiquitination, endocytosis, and lysosomal degradation of the IFNAR1 subunit of the type I  interferon (IFN) receptor is mediated by the SCFbeta-Trcp (Skp1-Cullin1-F-box protein beta transducin repeat-containing protein) E3 ubiquitin ligase in a phosphorylation-dependent manner. Stability of IFNAR1 is regulated by its binding to Tyk2 kinase. Expression of Tyk2 stabilized IFNAR1 in a manner that was dependent neither on its binding to beta-Trcp nor IFNAR1 ubiquitination.

References

1. Abramovich C., et al., 1994, FEBS Lett. 338:295-300.
2. Colamonici O., et al., 1994, Mol. Cell. Biol. 14:8133-42.
3. Kumar,K.G. et al., 2004, J Biol Chem  279 (45): 46614-20.
4. Gauci S., et al., 2009, Anal. Chem. 81:4493-501.
5. Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-86.
6. Mayya V., et al., 2009, Sci. Signal. 2: RA46-RA46.