IFNAR1 Antibody

IFNAR-1 Antibody Datasheet

IFNAR-1 Antibody Product Information

• Immunogen: Recombinant Mouse IFNAR-1 protein (Catalog#50469-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 114
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IFNAR1 (rM IFNAR1; Catalog#50469-M08H; NP_034638.2; Met 1-Thr 429).

IFNAR-1 Antibody Usage Guide

• Specificity: Mouse IFNAR-1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse IFNAR1 in WB. Using a DAB detection system, the detection limit for Mouse IFNAR1 is approximately 0.25 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IFNAR1. The detection limit for Mouse IFNAR1 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IFNAR-1 Antibody Background

Interferon alpha/beta receptor 1, also known as IFN-alpha/beta receptor 1, Type I interferon receptor 1, Cytokine receptor family 2 member 1, Cytokine receptor class-II member 1, CRF2-1, IFNAR1 and IFNAR, is a single-pass type I membrane protein which belongs to thetype II cytokine receptor family. IFNAR1 is a receptor for interferons alpha and beta which contains threefibronectin type-III domains. The binding of IFNAR1 to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform1, isoform2and isoform3are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform2and isoform3are expressed in the IFN-alpha resistant myeloma cell line U266R. Ubiquitination, endocytosis, and lysosomal degradation of the IFNAR1 subunit of the type I interferon (IFN) receptor is mediated by the SCFbeta-Trcp (Skp1-Cullin1-F-box protein beta transducin repeat-containing protein) E3 ubiquitin ligase in a phosphorylation-dependent manner. Stability of IFNAR1 is regulated by its binding to Tyk2 kinase. Expression of Tyk2 stabilized IFNAR1 in a manner that was dependent neither on its binding to beta-Trcp nor IFNAR1 ubiquitination.

References

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