Mouse IFNA2 / Interferon alpha 2 Protein

Interferon alpha 2 Protein Datasheet

IFNA2 / Interferon alpha 2 Protein Product Information

• Synonym: P23-139P14.4, Ifa2
• Protein Construction: A DNA sequence encoding the mouse IFNA2 (P01573) (Cys 24-Glu 190) was fused with the Fc region of human IgG1 at the N-terminus.
• Source: Mouse
• Expression Host: Human Cells

IFNA2 / Interferon alpha 2 Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu
• Molecular Mass: The recombinant mouse IFNA2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 427 amino acids and has a calculated molecular mass of 47.8 kDa. The apparent molecular mass of the monomer is approximately 52 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IFNA2 / Interferon alpha 2 protein

IFNA2 / Interferon alpha 2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFNA2 / Interferon alpha 2 Protein Description

Interferon alpha 2 (IFN-alpha 2) is a member of the IFN alpha protein family. The IFN alpha proteins are produced by leukocytes. They are mainly involved in innate immune response against viral infection. IFN alpha acts as a pyrogenic factor by altering the activity of thermosensitive neurons in the hypothalamus thus causing fever. It does this by binding to opioid receptors and eliciting the release of prostaglandin-E2 (PGE2). A similar mechanism is used by IFN alpha to reduce pain; IFN alpha interacts with the μ-opioid receptor to act as an analgesic. Interferon alpha 2 (IFN-alpha 2) was isolated from a preparation of partially purified human leucocyte IFN by monoclonal-antibody immunoaffinity chromatography. Interferon alpha-2 (IFN alpha 2) is a leukocyte product with several biological properties including antiviral activity, pyrogenicity and enhancement of immune functions. It has been reported that an additional facet of Interferon alpha-2 activity is its ability to enhance slow-wave sleep (SWS) without greatly altering other aspects of sleep. The sleep-promoting activity of Interferon alpha-2 may be related to feelings of lassitude and sleepiness that often accompany viral disease and interferon therapy. Recombinant alpha-2 interferon has been used in the treatment of Kaposi's sarcoma, and its toxicity and efficacy is associated with the acquired immunodeficiency syndrome.

References

1. Wang. et al., 2004, J Neuroimmunol. 156 (1-2): 107-12.
2. Groopman JE. et al., 1984, Ann Intern Med. 100 (5): 671-6.
3. Krueger JM. et al., 1987, Int J Immunopharmacol. 9 (1): 23-30.