Rat IFNA1 Gene cDNA Clone / ORF Clone

IFNA1 cDNA Clone Datasheet

IFNA1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001014786.1
• cDNA Size: 579
• Gene Synonym: IFN-alpha1, Ifna1
• Source: Rat

IFNA1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interferon-alpha 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IFNA1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IFNA1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IFNA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCGGC TCTGTGCTTT CTTGATGTCC CTGGTGGTGG TGAGCTACTG GTCAGCCTGC
61       TGTCTAGGAT GTGACCTGCC TCATACTCAT AACCTCAGGA ACAAGAGAGT CTTCACACTC
121      CTGGCACAAA TGAGGAGACT CTCCCCTGTC TCATGCCTGA AGGACAGAAA GTACTTTGGG
181      TTCCCTTTGG AGAAGGTGGA TGGCCAGCAG ATCCAGAAGG CTCAAGCTAT CCCTGTCCTG
241      CATGAGCTGA CCCAGCAGAT CCTCAGCCTC TTCACATCAA AGGAGTCATC TACTGCTTGG
301      GATGCAACCC TCCTAGACTC ATTCTGTAAT GACCTCCAGC AGCAGCTGAG TGGTCTGCAA
361      GCCTGTCTGA TGCAGCAGGT AGGGGTGCAG GAATCTCCCC TGACCCAGGA AGACTCCCTA
421      CTGGCTGTGA GGGAATACTT CCACAGAATC ACTGTGTACC TGAGAGAGAA TAAACACAGC
481      CCCTGTGCCT GGGAGGTGGT CAAAGCAGAA GTCTGGAGAG CCCTGTCTTC CTCAGCCAAC
541      TTGATGGGAA GACTGAGAGA AGAAAGAAAT GAGTCCTGA

 

Quality Control:cDNA sequencing results is available upon request.