Rat IFN-alpha / IFNA1 / IFN Protein (His Tag) (Cytokine)

Interferon-alpha 1 Protein Datasheet

IFN-alpha / IFNA1 / IFN Protein Product Information

• Synonym: IFNA1
• Protein Construction: A DNA sequence encoding the rat IFNA1 (P05011) (Met1-Ser192) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

IFN-alpha / IFNA1 / IFN Protein QC Testing

• Purity: > 99 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Cys 24
• Molecular Mass: The recombinant rat IFNA1 comprises 180 amino acids and predicts a molecular mass of 20.9 kDa. The apparent molecular mass of the ratIL20RB is approximately 21 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IFN-alpha / IFNA1 / IFN protein

IFN-alpha / IFNA1 / IFN Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFN-alpha / IFNA1 / IFN Protein Description

IFN (interferon-alpha 1,human IgG1-Fc chimera), also known as IFN-alpha, IFNA13 and IFNA1, belongs to the alpha/beta interferon family. IFNs belong to the large class of glycoproteins known as cytokines. Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon (IFN-gamma) is produced by mitogen- or antigen-stimulated antigen-stimulated T lymphocytes. IFNs are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells. Produced by macrophages, IFN have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFNs can activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they also increase the ability of uninfected host cells to resist new infection by virus.

References

1. Olopade OI. et al., 1992, Genomics. 14 (2): 437-43.
2. Conti L. et al., 2005, Immunobiology. 209 (1-2): 99-115.
3. Bekisz J. et al., 2005, Growth Factors. 22 (4): 243-51.