IFNα4 / IFNa4 / Interferon alpha-4 Protein (His Tag) (Cytokine)

Interferon, alpha 4 Protein Datasheet

IFNα4a / IFNa4a / Interferon alpha-4 Protein Product Information

• Synonym: INFA4, MGC142200
• Protein Construction: A DNA sequence encoding the human IFNα 4a (NP_066546.1)) (Met 1- Asp 189) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

IFNα4a / IFNa4a / Interferon alpha-4 Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE.
• Bio-activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV).
  The ED₅₀ for this effect is typically 2-8 pg/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Cys 24
• Molecular Mass: The recombinant human IFNα4a consists of 176 amino acids and predicts a molecular mass of 20.7 kDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IFNα4a / IFNa4a / Interferon alpha-4 protein

IFNα4a / IFNa4a / Interferon alpha-4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFNα4a / IFNa4a / Interferon alpha-4 Protein Description

Interferons (IFNs) are natural glycoproteins belonging to the cytokine superfamily, and are produced by a wide variety of cells types in response to challenges by foreign agents such as viruses, parasites and tumor cells. There are three major classes of IFNs according to the receptor type through which they signal, and the type I IFNs bind to a specific receptor complex known as the IFN-α receptor (IFNAR) consisting of IFNAR1 and IFNAR2 chains. The type I IFNs present in humans are IFN-α, IFN-β and IFN-ω, and the IFN-α come in 13 subtypes whose encoding genes locate in a cluster on chromosome 9. IFN-α is produced by leukocytes and mainly involved in innate immune response against viral infection together with other IFNs. IFN-α is produced in response to the presence of dsRNA, a key indicator of viral infection via Toll Like Receptors, and assists the immune response by inhibiting viral replication within host cells, activating natural killer cells and macrophages, increasing antigen presentation to lymphocytes, and inducing the resistance of host cells to viral infection. IFN-α also acts as a pyrogenic factor and thus causes fever by binding to opioid receptors and eliciting the release of prostaglandin-E2, and serves as an analgesic by employing the similar mechanism. Furthermore, IFN-α is suggested to promote apoptosis and accordingly has therapeutic effect against certain cancers by inducing increased production of the p53 gene product. Here, the IFN-α4a is an isoform of the human IFNA4 subtype.

References

1. Morikawa K. et al., 1987, J Immunol. 139: 761-6.
2. Sato K. et al., 2001, Eur J Immunol. 31: 3146-88.
3. Takaoka A. et al., 2003, Nature. 424: 516-23.
4. Hardy. et al., 2004, Genomics. 84: 331-45.
5. Pirhonen J. et al., 2007, J Leukoc Biol. 82: 1185-92.
6. Badr G. et al., 2005, Int Immunol. 17: 459-67.
7. Hussain M. et al., 1997, J Interferon Cytokine Res. 17: 559-66.