IFITM3 Protein

Interferon induced transmembrane protein 3 Protein Datasheet

IFITM3 Protein Product Information

• Synonym: IFITM3
• Protein Construction: A DNA sequence encoding the human IFITM3(NP_066362.2) (Met1-His57) was expressed, fused with the Fc region of mouse IgG₁ at the N-terminus.
• Source: Human
• Expression Host: Human Cells

IFITM3 Protein QC Testing

• Purity: > 99 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp
• Molecular Mass: The recombinant human IFITM3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 293 amino acids and has a predicted molecular mass of 32.9 kDa. The apparent molecular mass of the monomer is approximately 33-45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS,pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IFITM3 protein

IFITM3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IFITM3 Protein Description

Interferon-induced transmembrane protein 3 (IFITM3) belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM3 is both necessary and sufficient for preventing the emergence of viral genomes from the endosomal pathway. To replicate, viruses must gain access to the host cell's resources. Interferon (IFN) regulates the actions of a large complement of interferon effector genes (IEGs) that prevent viral replication. The interferon inducible transmembrane protein family members, IFITM1, 2 and 3, are IEGs required for inhibition of influenza A virus, dengue virus, and West Nile virus replication in vitro. Notably, viral pseudoparticles were inhibited from transferring their contents into the host cell cytosol by IFN, and IFITM3 was required and sufficient for this action. IFITM3 overexpression is sufficient for this phenotype. Moreover, IFITM3 partially resides in late endosomal and lysosomal structures, placing it in the path of invading viruses.

References

1. Tanaka SS. et al., 2005, Dev Cell. 9 (6): 745-56.
2. Li D. et al., 2011, Clin Cancer Res. 17 (11): 3558-68.
3. Lu J. et al., 2011, J Virol. 85 (5): 2126-37.