IFI30

IFI30 belongs to the GILT family. This family includes the two characterised human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL08. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. In addition, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulphide bond reduction. IFI30 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. IFI30 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing.

IFI30 Related Areas

Enzyme >>Other>> IFI30

IFI30 Alternative Names

GILT, IFI-30, IP30, MGC32056, gamma-interferon-inducible lysosomal thiol reductase, gamma-interferon-inducible protein IP-30, interferon gamma-inducible protein 30 preproprotein, legumaturain [Homo sapiens]

GILT, IP30, gamma-interferon-inducible lysosomal thiol reductase, gamma-interferon-inducible protein IP-30, lysosomal thio reductase IP30, lysosomal thiol reductase IP30 [Mus musculus]

Summaries for IFI30

Entrez Gene summary for IFI30:

The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing.

OMIM - description for IFI30:

Proteins internalized into the endocytic pathway are usually degraded. Efficient proteolysis requires denaturation, induced by acidic conditions within lysosomes, and reduction of inter- and intrachain disulfide bonds. Cytosolic reduction is mediated enzymatically by thioredoxin (TXN; 187700). Arunachalam et al. (2000) described a lysosomal thiol reductase, which they called GILT, that was optimally active at low pH and capable of catalyzing disulfide bond reduction both in vivo and in vitro. They found that the GILT gene encodes a predicted 261-amino acid protein composed of a 37-amino acid signal peptide and a 224-amino acid proform.

Wikipedia summary for IFI30:

Gamma-interferon-inducible lysosomal thiol reductase is an enzyme that in humans is encoded by the IFI30 gene. The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing.

Human IFI30 Protein General Information

 

• Protein names: Recommended name: Gamma-interferon-inducible lysosomal thiol reductase
• Sequence length: 250 AA.
• Domain: Redox-active center Signal
• Sequence similarities: Belongs to the GILT family.
• Post-translational modification: N-glycosylated. Sugar chains contain mannose-6-phosphate. Ref.2 Ref.8 Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.
• Subunit structure: Dimer; disulfide-linked
• Subcellular location: Secreted. Lysosome
• Sequence caution: The sequence AAA36105.1 differs from that shown. Reason: Erroneous termination at position 251. Translated as stop. The sequence AAA36105.1 differs from that shown. Reason: Frameshift at positions 146 and 174. The sequence AAA36105.1 differs from that shown. Reason: Chimeric cDNA. N-terminal sequence identical to a region of chromosome 11. The sequence AAF04618.1 differs from that shown. Reason: Chimeric cDNA. N-terminal sequence identical to a region of chromosome 11.

General information above from UniProt

Function for IFI30 Protein

UniProtKB:

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds By similarity. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation

Genatlas:

• IFI30 plays an important role in antigen processing
• IFI30 has an important role in MHC class II-restricted antigen processing
• GILT is responsible for the activation of haemolysin listeriolysin O in vivo
• IFI30 is facilitated the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds
• capable of catalyzing disulfide bond reduction both in vivo and in vitro

Homology for human IFI30

• ortholog to Ifi30, murine