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Rat ICOS Gene cDNA Clone / ORF Clone

Catalog Vector (Price) Quantity In Stock Operation
RG80381-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ICOS cDNA Clone Datasheet

 

ICOS cDNA Clone Product Information

Gene Bank Ref.ID: NM_022610.1

cDNA Size:

603

Gene Synonym:

Ailim; Icos

Source: Rat

ICOS cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus inducible T-cell co-stimulator DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ICOS cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ICOS cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ICOS cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGCCCT ACTTCTCGTG CGTCTTTGTC TTCTGCTTCC TAATCAAACT TTTAACAGGA
61       GAACTCAATG ACTTGGCCAA TCACAGGATG TTTTCGTTTC ACGATGGAGG TGTACAGATT
121      TCTTGTAACT ACCCTGAGAC TGTCCAGCAG TTAAAAATGC AGTTGTTCAA AGACAGAGAA
181      GTCCTCTGCG ACCTCACCAA GACCAAGGGA AGCGGAAACA CCGTGTCCAT CAAGAATCCG
241      ATGTCCTGTC CATATCAGCT GTCCAACAAC AGTGTCTCTT TTTTCCTAGA CAACGCAGAC
301      AGCTCCCAGG GCAGCTACTT TTTATGCAGC CTGTCGATTT TCGACCCACC CCCTTTTCAA
361      GAAAAGAACC TTAGTGGAGG ATATTTGCTT ATTTATGAAT CCCAGCTTTG TTGCCAGCTG
421      AAGCTTTGGT TACCCGTAGG GTGTGCAGCT TTTGTGGCAG CGCTCCTTTT TGGATGCATA
481      TTTATCGTCT GGTTTGCAAA AAAGAAGTAC AGATCCAGTG TGCACGACCC TAATAGCGAG
541      TACATGTTCA TGGCGGCAGT CAACACAAAC AAAAAGTCCA GACTTGCAGG TATGACCTCA
601      TAA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

CD278 (ICOS/AILIM) related areas, pathways, and other information