|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ICOS ORF mammalian expression plasmid, C-GFPSpark tag||HG10344-ACG|
|Human ICOS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10344-ACR|
|Human ICOS ORF mammalian expression plasmid, C-Flag tag||HG10344-CF|
|Human ICOS ORF mammalian expression plasmid, C-His tag||HG10344-CH|
|Human ICOS ORF mammalian expression plasmid, C-Myc tag||HG10344-CM|
|Human ICOS ORF mammalian expression plasmid, C-HA tag||HG10344-CY|
|Human ICOS ORF mammalian expression plasmid, N-Flag tag||HG10344-NF|
|Human ICOS ORF mammalian expression plasmid, N-His tag||HG10344-NH|
|Human ICOS ORF mammalian expression plasmid, N-Myc tag||HG10344-NM|
|Human ICOS ORF mammalian expression plasmid, N-HA tag||HG10344-NY|
|Human ICOS natural ORF mammalian expression plasmid||HG10344-UT|
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Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) is a cell-surface receptor, and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T−B cell interactions which is essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS is more potent in the induction of IL-10 production, a cytokine important for suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provides key second signals that can regulate the activation, inhibition and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested of being involved in the development of airway inflammation and airway hyperresponsiveness.