Human ICOS Gene cDNA Clone / ORF Clone

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_012092.2

cDNA Size:   600

Synonym:  AILIM, CD278, MGC39850

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens inducible T-cell co-stimulator  DNA.

Vector:  Vector pMD18-T Simple

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGAAGTCAG GCCTCTGGTA TTTCTTTCTC TTCTGCTTGC GCATTAAAGT TTTAACAGGA
61       GAAATCAATG GTTCTGCCAA TTATGAGATG TTTATATTTC ACAACGGAGG TGTACAAATT
121      TTATGCAAAT ATCCTGACAT TGTCCAGCAA TTTAAAATGC AGTTGCTGAA AGGGGGGCAA
181      ATACTCTGCG ATCTCACTAA GACAAAAGGA AGTGGAAACA CAGTGTCCAT TAAGAGTCTG
241      AAATTCTGCC ATTCTCAGTT ATCCAACAAC AGTGTCTCTT TTTTTCTATA CAACTTGGAC
301      CATTCTCATG CCAACTATTA CTTCTGCAAC CTATCAATTT TTGATCCTCC TCCTTTTAAA
361      GTAACTCTTA CAGGAGGATA TTTGCATATT TATGAATCAC AACTTTGTTG CCAGCTGAAG
421      TTCTGGTTAC CCATAGGATG TGCAGCCTTT GTTGTAGTCT GCATTTTGGG ATGCATACTT
481      ATTTGTTGGC TTACAAAAAA GAAGTATTCA TCCAGTGTGC ACGACCCTAA CGGTGAATAC
541      ATGTTCATGA GAGCAGTGAA CACAGCCAAA AAATCTAGAC TCACAGATGT GACCCTATAA

Quality Control:   cDNA sequencing results is available upon request.