Mouse ICOS / AILIM / CD278 Protein

Inducible T-cell co-stimulator Protein Datasheet

ICOS/ AILIM / CD278 Protein Product Information

• Synonym: CH29-120A16.4, AILIM, CCLP, CRP-1, H4, Ly115
• Protein Construction: A DNA sequence encoding the mouse ICOS (Q9WVS0) extracellular domain (Met 1-Leu 142) was fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

ICOS/ AILIM / CD278 Protein QC Testing

• Purity: > 97% as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 21
• Molecular Mass: The recombinant mouse ICOS/Fc is a disulfide-linked homodimer. The reduced monomer consists of 363 amino acids and has a predicted molecular mass of 40.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmICOS/Fc monomer is approximately 40-45 KDa.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ICOS/ AILIM / CD278 protein

ICOS/ AILIM / CD278 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ICOS/ AILIM / CD278 Protein Description

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) is a cell-surface receptor, and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. Optimal T cell activation and expansion are regulated by signals delivered not only through the T cell receptor (TCR), but also a number of costimulatory molecules. ICOS is expressed on antigen-primed T cells, and B7RP-1(or B7-H2) on B cells and macrophages has been identified as the ICOS ligand. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T?B cell interactions which is essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS also regulates TH2-mediated mucosal inflammatory responses partially through the signal for up-regulation of the chemokine receptors, and accordingly, aberrant expression of ICOS is implicated in allergic inflammatory responses and other immunity disorders.

References

1. Hutloff, A. et al., 1999, Nature, 397: 263-6.
2. Yoshinaga, S. K. et al. 1999, Nature, 402: 827-32.
3. Gonzalo, J.A. et al., 2001, Nat. Immunol. 2: 597-604.
4. Coyle, A.J. et al., 2000, Immunity. 13: 95-105.
5. Dong, C. et al. 2001, Nature. 409: 97-101.
6. Aicher, A. et al., 2000, J. Immunol. 164: 4689-96.