ICAM1 / CD54 Antibody

ICAM1 / CD54 Antibody Datasheet

ICAM1 / CD54 Antibody Product Information

• Immunogen: Recombinant Human ICAM1 / CD54 protein (Catalog#10346-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 01
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 (rh ICAM1 / CD54; Catalog#10346-H08H; Met 1-Glu 480; NP_000192.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ICAM1 / CD54 Antibody Usage Guide

• Specificity: Human ICAM1 / CD54
• No cross-reactivity in ELISA with
  

  Human ICAM2

  Human ICAM3

  Human VCAM1

  Human CD62E

  Human CEACAM1

  Human cell lysate (293 cell line)

• Flow Cytometry:
  

  Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were either treated with TNF-α (50 ng/ml, 18 hours) (right panel) or untreated (left panel). The cells were harvested and stained with 1 μl purified human ICAM1 (10346-MM01, 1 μg/test), then a FITC-conjugated second step antibody.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ICAM1.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ICAM1 / CD54 Antibody Background

ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule constitutely present in low concentrations in the membranes of leukocytes and endothelial cells. As an endothelial and leukocyte-associated transmembrane protein, ICAM1 is well known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration.ICAM1 is a ligand for LFA-1 (integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease.

References

1. Rothlein, R. et al.,1986, Journal of Immunology. 137 (4): 1270-1274.
2. Yang, L. et al., 2005, Blood. 106 (2): 584-592.
3. Frijns, C.J. et al., 2002, Stroke. 33 (8): 2115-2122.
4. Bella, J.et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95 (8): 4140-4145.