Anti-ICAM-2 Antibody

Cat: 10332-RP02
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Anti-ICAM-2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ICAM-2 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human ICAM-2
Immunogen
Recombinant Human ICAM-2 / CD102 protein (Catalog#10332-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human ICAM-2 / CD102 (rh ICAM-2 / CD102; Catalog#10332-H08H; NP_777596.2; Met 1-Gln 223). ICAM-2 / CD102 specific IgG was purified by Human ICAM-2 / CD102 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ICAM-2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ICAM-2 Antibody (Rabbit Polyclonal antibody) Images
Human ICAM-2/CD102 Immunoprecipitation(IP) 6160

ICAM2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-ICAM2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-ICAM2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 31 kDa

Observed band size: 31 kDa

Human ICAM-2/CD102 Western blot (WB) 13186

Anti-ICAM2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HL60 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:47 kDa

Anti-ICAM-2 Antibody Alternative Names
Anti-CD102 Antibody
ICAM-2 Background Information

Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.

Full Name
intercellular adhesion molecule 2
References
  • Weber KS, et al. (2004) Sialylation of ICAM-2 on platelets impairs adhesion of leukocytes via LFA-1 and DC-SIGN. Inflammation. 28(4): 177-88.
  • Tanaka H, et al. (2004) ICAM-2 gene therapy for peritoneal dissemination of scirrhous gastric carcinoma. Clin Cancer Res. 10(14): 4885-92.
  • Younis AI, et al. (2005) Intercellular adhesion molecule-2 (ICAM-2) in experimental trichinosis. J Egypt Soc Parasitol. 35(3): 1019-26.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ICAM-2 Antibody Rabbit Monoclonal WB,ELISA 10332-R041
    Anti-ICAM-2 Antibody Rabbit Monoclonal WB 10332-R055
    Anti-ICAM-2 Antibody Mouse Monoclonal ELISA 10332-MM01
    Anti-ICAM-2 Antibody Rabbit Monoclonal ELISA(Det) 10332-R015
    Anti-ICAM-2 Antibody Mouse Monoclonal IHC-P 10332-MM06
    Anti-ICAM-2 Antibody Rabbit Monoclonal WB 10332-R033
    Anti-ICAM-2 Antibody Rabbit Polyclonal ELISA 10332-RP01
    Anti-ICAM-2 Antibody Rabbit Monoclonal ELISA(Cap) 10332-R022
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