ICAM2 / CD102 Antibody

Intercellular Adhesion Molecule 2 ( ICAM2 / CD102 ) Antibody

ICAM2 / CD102 Antibody Product Information

• Immunogen: Recombinant human ICAM2 protein ( Catalog#10332-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 041
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human ICAM2 ( rh ICAM2 ; Catalog#10332-H08H ; NP_777596.2 ; Met 1 - Gln 223).

ICAM2 / CD102 Antibody Usage Guide

• Specificity: Human ICAM2 / CD102
• No cross-reactivity in ELISA with

  Human CEACAM1 / CD66a

  Human ICAM1 / CD54

  Human ICAM3 / CD50

  Human CD31 / PECAM1

  Mouse VCAM1 / L1CAM / CD106

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human ICAM2 in WB. Using a DAB detection system, the detection limit for human ICAM2 is approximately 0.125 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human ICAM2. The detection limit for human ICAM2 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ICAM2 / CD102 Antibody Background

Intercellular adhesion molecule 2 ( ICAM2, CD102 ), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I  transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 ( CD11a/CD18 ) and Mac-1 ( CD11b/CD18 ). ICAM2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocyte with other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. This protein can be encoded by several transcript variants.

References

1. Staunton, D.E. et al., 1989, Nature. 339: 61-64.
2. Simmons, D.L. et al., 1995, Cancer. Surv. 24: 141-155.
3. Fagerholm, S.C. et al., 2006, Blood. 108: 3379-3386.
4. Issekutz, A.C. et al., 1999, J. Leukoc. Biol. 65: 117-126.
5. Somersalo, K. et al., 1995. J. Biol. Chem. 270: 8629-8636.