ICAM-1 / CD54 Antibody

ICAM-1 / CD54 Antibody Datasheet

ICAM-1 / CD54 Antibody Product Information

• Immunogen: Recombinant Rat ICAM-1 / CD54 protein (Catalog#80022-R08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 2D7E7
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat ICAM-1 / CD54 (rR ICAM-1 / CD54; Catalog#80022-R08H; Q00238-1; Met 1-Thr 493). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ICAM-1 / CD54 Antibody Usage Guide

• Specificity: Rat ICAM-1 / CD54
• No cross-reactivity in ELISA with
  Human CD31

  Human ICAM1

  Mouse ICAM1

  Human ICAM3

  Mouse VCAM1

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rat ICAM1. The detection limit for Rat ICAM1 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ICAM-1 / CD54 Antibody Background

ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule constitutely present in low concentrations in the membranes of leukocytes and endothelial cells. As an endothelial and leukocyte-associated transmembrane protein, ICAM1 is well known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration.ICAM1 is a ligand for LFA-1 (integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease.

References

1. Rothlein, R. et al.,1986, Journal of Immunology. 137 (4): 1270-1274.
2. Yang, L. et al., 2005, Blood. 106 (2): 584-592.
3. Frijns, C.J. et al., 2002, Stroke. 33 (8): 2115-2122.
4. Bella, J.et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95 (8): 4140-4145.