ICAM-1 / CD54 Antibody ( FITC )

ICAM-1 / CD54 Antibody Datasheet

ICAM-1 / CD54 Antibody Product Information

• Immunogen: Recombinant Human ICAM-1 protein (Catalog#10346-H08H)
• Reagents: FITC-conjugated mouse monoclonal antibody
• Clone ID: 01
• Ig Type: Mouse IgG1
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM-1 / CD54 (rh ICAM-1 / CD54; Catalog#10346-H08H; NP_000192.2; Met 1-Glu 480) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

ICAM-1 / CD54 Antibody Usage Guide

• Specificity: Human ICAM-1 / CD54
• No cross-reactivity in ELISA with
  

  Human ICAM2

  Human ICAM3

  Human VCAM1

  Human CD62E

  Human CEACAM1

  Human cell lysate (293 cell line)

• Flow Cytometry:
  

  Profile of anti-ICAM1(CD54) reactivity on Daudi cells analyzed by flow cytometry.

  

  Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were treated with TNF-α (50 ng/ml,18 hours). The cells were harvested and stained with FITC Mouse anti-Human ICAM1 (10346-MM01).

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

ICAM-1 / CD54 Antibody Background

ICAM1 (Inter-Cellular Adhesion Molecule 1), also known as CD54, is a type of intercellular adhesion molecule constitutely present in low concentrations in the membranes of leukocytes and endothelial cells. As an endothelial and leukocyte-associated transmembrane protein, ICAM1 is well known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration.ICAM1 is a ligand for LFA-1 (integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease.

References

1. Rothlein, R. et al.,1986, Journal of Immunology. 137 (4): 1270-1274.
2. Yang, L. et al., 2005, Blood. 106 (2): 584-592.
3. Frijns, C.J. et al., 2002, Stroke. 33 (8): 2115-2122.
4. Bella, J.et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95 (8): 4140-4145.