Anti-ICAM-1 Antibody

Cat: 80022-RP02
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Anti-ICAM-1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ICAM-1 Antibody
Validated applications
ELISA,IP
Species reactivity
Reacts with: Rat
Specificity
Rat ICAM-1
Immunogen
Recombinant Rat ICAM-1 / CD54 protein (Catalog#80022-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat ICAM-1 / CD54 (rR ICAM-1 / CD54; Catalog#80022-R08H; Q00238-1; Met 1-Thr 493). ICAM-1 / CD54 specific IgG was purified by Rat ICAM-1 / CD54 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ICAM-1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ICAM-1 Antibody (Rabbit Polyclonal antibody) Images
Rat ICAM-1/CD54 Immunoprecipitation(IP) 8266

rat ICAM1 was immunoprecipitated using:

Lane A:0.5 mg Raw264.7 Whole Cell Lysate

2 µL anti-rat ICAM1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-rat ICAM1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size: 116 kDa

ICAM-1 Background Information

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

Full Name
intercellular adhesion molecule 1
Research Areas
References
  • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
  • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
  • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
  • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ICAM-1 Antibody (FITC) Rabbit Monoclonal FCM 80022-R037-F
    Anti-ICAM-1 Antibody Rabbit Monoclonal ELISA(Cap),FCM,ICC/IF,IF 80022-R037
    Anti-ICAM-1 Antibody Rabbit Monoclonal IHC-P 80022-R003
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA 80022-RP01
    Anti-ICAM-1 Antibody Rabbit Monoclonal ELISA 80022-R143
    Anti-ICAM-1 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 80022-R112
    Anti-ICAM-1 Antibody (PE) Rabbit Monoclonal FCM 80022-R037-P
    Anti-ICAM-1 Antibody Mouse Monoclonal ELISA 80022-MM11
    Anti-ICAM-1 Antibody (APC) Rabbit Monoclonal FCM 80022-R037-A
    Anti-ICAM-1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 80022-T60
    Anti-ICAM-1 Antibody Rabbit Monoclonal IHC-P 50440-R280
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA,IHC-P 50440-T24
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA 50440-RP01
    Anti-ICAM-1 Antibody Rabbit Monoclonal ELISA(Det) 50440-R101
    Anti-ICAM-1 Antibody Rabbit Monoclonal ELISA,ELISA(Cap),IHC-P 50440-R020
    Anti-ICAM-1 Antibody Rabbit Monoclonal FCM 50440-R009
    Anti-ICAM-1 Antibody (PE) Rabbit Monoclonal FCM 50440-R009-P
    Anti-ICAM-1 Antibody (FITC) Rabbit Monoclonal FCM 50440-R009-F
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA,IHC-P 50440-RP02
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA 10346-T16
    Anti-ICAM-1 Antibody (PE) Mouse Monoclonal FCM 10346-MM01-P
    Anti-ICAM-1 Antibody (PerCP) Mouse Monoclonal FCM 10346-MM01-C
    Anti-ICAM-1 Antibody (FITC) Mouse Monoclonal FCM 10346-MM01-F
    Anti-ICAM-1 Antibody Mouse Monoclonal ELISA,ELISA(Cap),FCM,ICC/IF,IF 10346-MM01
    Anti-ICAM-1 Antibody Mouse Monoclonal ELISA 10346-MM04
    Anti-ICAM-1 Antibody (APC) Mouse Monoclonal FCM 10346-MM01-A
    Anti-ICAM-1 Antibody Mouse Monoclonal WB 10346-MM03
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA,IHC-P,ICC/IF,IF 10346-T26
    Anti-ICAM-1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 10346-RP02
    Anti-ICAM-1 Antibody Rabbit Monoclonal ELISA 10346-R013
    Anti-ICAM-1 Antibody Rabbit Polyclonal ELISA 10346-RP01
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