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ICAM-1 / CD54 Antibody, Mouse MAb

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Human ICAM1 Antibody Product Information
Immunogen:Recombinant Human ICAM-1 protein (Catalog#10346-H08H)
Clone ID:4G1B2
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 extracellular domain (rh ICAM1; Catalog#10346-H08H; NP_000192.2; Met 1-Glu 480). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human ICAM1 Antibody Usage Guide
Specificity:Human ICAM1 / CD54
No cross-reactivity in WB and ELISA with
Human ICAM2 / CD102
Human ICAM3 / CD50,Human VCAM1 / CD106
Human CEACAM1 / CD66a
Human E-selectin / SELE / CD62E / ELAM1
Human cell lysate (293 cell line)
Application:ELISA

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ICAM1. The detection limit for Human ICAM1 is 0.16 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other ICAM1 Antibody Products
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      10346-R013-50
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        50440-R009-F-50
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            80022-MM11-50
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              Application: FCM  
              • Slide 1
              80022-R037-A-50
              80022-R037-A-100
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              100 Tests 
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              Reactivity: Rat  
              Application: IHC-P  
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              80022-MM01-100
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              80022-MM01-200
              100 µg 
              50 µg 
              200 µg 
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              Reactivity: Rat  
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              ICAM-1/CD54 Background

              Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

              Human ICAM-1/CD54 References
            • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
            • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
            • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
            • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.
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              Catalog: 10346-MM04-50
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              All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
              Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"